Human TIAL1 knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
TIAL1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and Insertion of the selection cassette in exon2.
View Alternative Names
Aging associated gene 7 protein, Nucleolysin TIAR, T cluster binding protein, TCBP, TIA 1 related nucleolysin, TIA-1-related protein, TIA1 cytotoxic granule associated RNA binding protein like 1, TIAR_HUMAN
- WB
Lab
Western blot - Human TIAL1 knockout HeLa cell lysate (AB257743)
Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : TIAL1 knockout HeLa cell lysate (20 ug)
Lane 3 : Jurkat cell lysate (20 ug)
Lane 4 : K-562 cell lysate (20 ug)
ab169547 was shown to specifically react with TIAL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265793 (knockout cell lysate ab257743) was used. Wild-type and TIAL1 knockout samples were subjected to SDS-PAGE. ab169547 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-TIAL1 antibody [EPR11323(B)] (<a href='/en-us/products/primary-antibodies/tial1-antibody-epr11323b-ab169547'>ab169547</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
TIAL1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human TIAL1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-tial1-knockout-hela-cell-line-ab265793'>ab265793</a>)
Lane 3:
Jurkat cell lysate at 20 µg
Lane 4:
K-562 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 41 kDa
Observed band size: 49 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human TIAL1 knockout HeLa cell lysate (AB257743)
Allele-1 : 1 bp insertion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human TIAL1 knockout HeLa cell lysate (AB257743)
Allele-3 : Insertion of the selection cassette in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human TIAL1 knockout HeLa cell lysate (AB257743)
Allele-2 : Insertion of the selection cassette in exon2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TIAL1 influences gene expression by associating with stress granules cytoplasmic aggregates formed in response to cellular stress. This association helps inhibit translation initiation modulating the cellular stress response. TIAL1 is part of a complex with other RNA-binding proteins that integrate signaling events and manage mRNA turnover and stress granule dynamics. Through this interaction it affects apoptosis and contributes to cell survival under stress conditions.
Pathways
Studies demonstrate that TIAL1 plays a role in the apoptotic signaling pathway and the stress response pathway. By interacting with proteins such as eIF2α kinase TIAL1 helps mediate the cellular response to environmental and oxidative stress. These pathways maintain cellular homeostasis and TIAL1 assists in regulating translation during stress ensuring proper cell function.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAL1 antibody [EPR11323(B)] (AB169547)](https://content.abcam.com/products/images/tial1-antibody-epr11323b-ab169547--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125861.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com