Human TIMM17A (TIM17) knockout HEK-293T cell lysate
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TIMM17A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.
View Alternative Names
Inner membrane preprotein translocase Tim17a, Mitochondrial import inner membrane translocase subunit Tim17-A, TI17A_HUMAN, TIM17, TIM17A, TIMM17A, mitochondrial inner membrane translocase, preprotein translocase, translocase of inner mitochondrial membrane 17 homolog A (yeast)
- Sanger seq
Unknown
Sanger Sequencing - Human TIMM17A (TIM17) knockout HEK-293T cell lysate (AB258230)
Allele-1 : Insertion of the selection cassette in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human TIMM17A (TIM17) knockout HEK-293T cell lysate (AB258230)
Allele-2 : 11 bp deletion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The TIMM17A protein facilitates the import of essential mitochondrial proteins securing proper mitochondrial function and energy production. TIMM17A as part of the TIM23 translocase complex cooperates closely with other TIM proteins such as TIMM50 and TIMM23 to ensure protein import is efficient and targeted. Its role in maintaining mitochondrial integrity also supports cellular metabolism and the regulation of apoptotic processes which are processes responsible for programmed cell death.
Pathways
The TIMM17A protein plays a significant role in the mitochondrial protein import pathway. It interacts with other key proteins like HSP70 and mtHSP70 which manipulate peptide folding and assist in pulling preproteins through the TIM23 channel. The proper import of these proteins is essential for the oxidative phosphorylation pathway which is important for ATP production. This pathway enables ATP synthesis by transferring electrons through a sequence of electron transport chain complexes located in the mitochondrial inner membrane.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-TIMM17A/TIM17 antibody [EPR16098] (AB192246)](https://content.abcam.com/products/images/timm17a-tim17-antibody-epr16098-ab192246--western-blot-img64084.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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