Human TIMM17B knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human TIMM17B knockout HEK-293T cell lysate (AB259179)
Allele-2 : 13 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human TIMM17B knockout HEK-293T cell lysate (AB259179)
Allele-1 : Insertion of the selection cassette in exon3
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TIMM17B integrates its function into the import machinery for mitochondrial proteins. It is part of the TIM22 complex an important multi-protein system that mediates the insertion and assembly of certain hydrophobic proteins into the mitochondrial inner membrane. TIMM17B works in coordination with other TIMM proteins such as TIM22 to facilitate the transfer and integration of mitochondrial carrier proteins. This integration supports the effective maintenance of mitochondrial functions which are essential for cellular energy metabolism.
Pathways
TIMM17B plays a significant role in the mitochondrial protein import pathway. This process involves the transport of proteins synthesized in the cytosol into the mitochondria which is essential for mitochondrial biogenesis and function. TIMM17B is also linked with the oxidative phosphorylation pathway through its involvement in maintaining mitochondrial membrane potential. Proteins like TIMM23 and TIMM50 are closely related to TIMM17B within these pathways providing important support for its function in importing proteins necessary for energy production.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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