Human TLE1 knockout MCF7 cell lysate
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TLE1 KO cell lysate available now. KO validated by Next Generation Sequencing. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9; X = 1 bp insertion; Frameshift = 100%.
View Alternative Names
E(Sp1) homolog, ESG, ESG1, Enhancer of split groucho 1, Enhancer of split groucho-like protein 1, GRG1, TLE1_HUMAN, Transducin like enhancer of split 1, Transducin like enhancer of split 1 (E(sp1) homolog Drosophila), Transducin like enhancer of split 1 homolog of Drosophila E(sp1), Transducin-like enhancer protein 1
- WB
Lab
Western blot - Human TLE1 knockout MCF7 cell lysate (AB269660)
Lane 1 : Wild-type MCF7 (Human breast adenocarcinoma cell line) whole cell lysate 20 ug
Lane 2 : TLE1 knockout MCF7 (Human breast adenocarcinoma cell line) whole cell lysate 20 ug
Lane 3 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate 20 ug
Lane 4 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate 20 ug
Lanes 1 - 4 : Merged signal (red and green). Green - ab183742 observed at 83 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab183742 was shown to react with TLE 1 in western blot. The band observed in the knockout cell line ab269498 (knockout cell lysate ab269660) lane below 83 kDa is likely to represent a truncated form. This has not been investigated further. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab183742 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes:
Western blot - Anti-TLE 1 antibody [EPR9386(2)] (<a href='/en-us/products/primary-antibodies/tle-1-antibody-epr93862-ab183742'>ab183742</a>) at 1/1000 dilution
Lane 1:
Wild-type MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 2:
TLE1 knockout MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 3:
SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg
Lane 4:
Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
Predicted band size: 83 kDa
Observed band size: 83 kDa
false
- NGS
Supplier Data
Next Generation Sequencing - Human TLE1 knockout MCF7 cell lysate (AB269660)
Knockout achieved by CRISPR/Cas9; X = 1 bp insertion; Frameshift = 100%
Reactivity data
Product details
Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The TLE1 protein plays a role in embryonic development by regulating the transcription of genes critical for cell differentiation. TLE1 forms part of a complex with other proteins to exert its corepressive function effectively. Through these interactions TLE1 is involved in differentiation processes in cells such as MCF-7 a breast cancer cell line influencing cellular behavior and function.
Pathways
TLE1 influences the Notch and Wnt signaling pathways essential for cell fate determination and proliferation. Its interaction with key proteins like β-catenin and Notch receptor proteins connects it to these pathways modulating the transcription of target genes. TLE1's role in these pathways is important for maintaining balanced cellular processes and preventing abnormal cell growth.
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB183742
Anti-TLE 1 antibody [EPR9386(2)]
BOND RX™ Validated|RabMAb|Recombinant|KO Validated
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE 1 antibody [EPR9386(2)] (AB183742)](https://content.abcam.com/products/images/tle-1-antibody-epr93862-ab183742--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img422679.jpg)
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Product promise
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