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TLE1 KO cell lysate available now. KO validated by Next Generation Sequencing. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9; X = 1 bp insertion; Frameshift = 100%.
Alternative names=E(Sp1) homolog, ESG, ESG1, Enhancer of split groucho 1, Enhancer of split groucho-like protein 1, GRG1, TLE1_HUMAN, Transducin like enhancer of split 1, Transducin like enhancer of split 1 (E(sp1) homolog Drosophila), Transducin like enhancer of split 1 homolog of Drosophila E(sp1), Transducin-like enhancer protein 1
- Cell type
MCF7
- Species or organism
Human
- Tissue
Breast
- Knockout validation
Next Generation Sequencing
Knockout achieved by CRISPR/Cas9; X = 1 bp insertion; Frameshift = 100%
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TLE1 KO cell lysate available now. KO validated by Next Generation Sequencing. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9; X = 1 bp insertion; Frameshift = 100%.
Alternative names=E(Sp1) homolog, ESG, ESG1, Enhancer of split groucho 1, Enhancer of split groucho-like protein 1, GRG1, TLE1_HUMAN, Transducin like enhancer of split 1, Transducin like enhancer of split 1 (E(sp1) homolog Drosophila), Transducin like enhancer of split 1 homolog of Drosophila E(sp1), Transducin-like enhancer protein 1
Key facts
- Cell type
MCF7
- Species or organism
Human
- Tissue
Breast
- Knockout validation
Next Generation Sequencing
- Mutation description
Knockout achieved by CRISPR/Cas9; X = 1 bp insertion; Frameshift = 100%
- Disease
Adenocarcinoma
- Concentration
Properties
- Gene name
TLE1
- Gene editing type
Knockout
- Gene editing method
CRISPR technology
- Knockout validation
Next Generation Sequencing
Cell culture
- Biosafety level
EU: 1 US: 1
- Adherent/suspension
Adherent
- Gender
Female
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
-20°C
Notes
Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
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