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AB258231

Human TLK1 knockout HeLa cell lysate

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TLK1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4 and Insertion of the selection cassette in exon 4.

View Alternative Names

PKU-beta, SNARE protein kinase SNAK, Serine threonine protein kinase, Serine/threonine-protein kinase tousled-like 1, TLK1_HUMAN, Tousled-like kinase 1

3 Images
Western blot - Human TLK1 knockout HeLa cell lysate (AB258231)
  • WB

Lab

Western blot - Human TLK1 knockout HeLa cell lysate (AB258231)

Lane 1 : Wild-type HeLa cell lysate 20 ug
Lane 2 : TLK1 knockout HeLa cell lysate 20 ug
Lane 3 : HL-60 cell lysate 20 ug
Lane 4 : PC3 cell lysate 20 ug
Lanes 1 - 4 : Merged signal (red and green). Green - Rabbit polyclonal antibody observed at 90 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
Rabbit polyclonal antibody was shown to react with TLK1 in wild-type HeLa cells in Western blot with loss of signal observed in TLK1 knockout cell line ab264898 (TLK1 knockout cell lysate ab258231). Wild-type HeLa and TLK1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with Rabbit polyclonal antibody and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Rabbit polyclonal antibody at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

TLK1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human TLK1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-tlk1-knockout-hela-cell-line-ab264898'>ab264898</a>)

Lane 3:

HL-60 cell lysate at 20 µg

Lane 4:

PC3 cell lysate at 20 µg

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Sanger Sequencing - Human TLK1 knockout HeLa cell lysate (AB258231)
  • Sanger seq

Unknown

Sanger Sequencing - Human TLK1 knockout HeLa cell lysate (AB258231)

Allele-2 : Insertion of the selection cassette in exon 4

Sanger Sequencing - Human TLK1 knockout HeLa cell lysate (AB258231)
  • Sanger seq

Unknown

Sanger Sequencing - Human TLK1 knockout HeLa cell lysate (AB258231)

Allele-1 : 1 bp insertion in exon 4

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4 and Insertion of the selection cassette in exon 4.

Disease

Adenocarcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
TLK1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Toutatis-like kinase 1 (TLK1) also known as serine/threonine-protein kinase tousled-like 1 is a kinase that plays a role in DNA repair and chromatin assembly. Its molecular mass is approximately 85 kDa. It is widely expressed in various human tissues with notable expression in the testis and brain. TLK1 primarily functions during DNA replication where it phosphorylates substrates that are vital for maintaining genomic stability.
Biological function summary

TLK1 contributes significantly to chromatin remodeling and DNA repair by influencing histone dynamics. TLK1 often acts in concert with other proteins as part of regulatory complexes modulating DNA accessibility during replication and repair processes. As a kinase it phosphorylates and activates targets like Asf1 a histone chaperone impacting nucleosome assembly.

Pathways

TLK1 plays a significant role in the DNA damage response and cell cycle regulation pathways. It is closely related to the ATM/ATR pathway which is central to the cellular response to DNA damage. TLK1 also interacts with proteins like Asf1 influencing replication-coupled chromatin assembly and cell cycle progression by modifying histones and other structural proteins.

TLK1 has been linked to certain cancers and neurodegenerative disorders. In cancer disruptions in TLK1 activity can lead to genomic instability contributing to tumorigenesis. TLK1 also connects with proteins like ASF1A in these contexts affecting cell proliferation and survival. In neurodegenerative diseases alterations in TLK1 function might impact neurological pathways related to DNA repair though specific mechanisms remain under investigation.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Proteins & Peptides

AB125563

Recombinant human TLK1 protein

Functional Studies - Recombinant human TLK1 protein (AB125563)

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Cell Lines & Lysates

AB264898

Human TLK1 knockout HeLa cell line

Advanced Validation

Western blot - Human TLK1 knockout HeLa cell line (AB264898)

  • 0
  • 0

Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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