TM4SF1 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 0 bp insertion in exon 1 and 1 bp insertion in exon 1.
Images
Key facts
- Cell type
HeLa
- Species or organism
Human
- Tissue
Cervix
- Knockout validation
Sanger Sequencing
- Mutation description
Knockout achieved by using CRISPR/Cas9, 0 bp insertion in exon 1 and 1 bp insertion in exon 1.
Alternative names
M3S1, Membrane component chromosome 3 surface marker 1, T4S1_HUMAN, TAAL6, Tm4sf1, Transmembrane 4 L6 family member 1, Tumor-associated antigen L6
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TM4SF1 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 0 bp insertion in exon 1 and 1 bp insertion in exon 1.
Key facts
- Cell type
HeLa
- Species or organism
Human
- Tissue
Cervix
- Knockout validation
Sanger Sequencing
- Mutation description
Knockout achieved by using CRISPR/Cas9, 0 bp insertion in exon 1 and 1 bp insertion in exon 1.
- Disease
Adenocarcinoma
- Concentration
Properties
- Gene name
TM4SF1
- Gene editing type
Knockout
- Gene editing method
CRISPR technology
- Knockout validation
Sanger Sequencing
Quality control
- STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
EU: 2 US: 2
- Adherent/suspension
Adherent
- Gender
Female
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Transmembrane 4 L6 family member 1 also called TM4SF1 is a protein with an approximate mass of 23 kDa. It is a member of the tetraspanin superfamily and consists of four transmembrane domains. TM4SF1 is mostly expressed in epithelial and endothelial cells with higher levels seen in many cancerous tissues. This protein integrates into cell membranes where it plays a role in cellular communication by interacting with other membrane proteins.
- Biological function summary
TM4SF1 is involved in regulating diverse cellular processes such as proliferation migration and angiogenesis. It is not part of any larger complex but works through its interactions with integrins growth factor receptors and other tetraspanins. The presence of TM4SF1 impacts the organization of lipid rafts facilitating cell signaling pathways essential for cell growth and survival.
- Pathways
TM4SF1 functions in both the PI3K/AKT and MAPK signaling cascades. These pathways are essential for controlling cell proliferation and survival. TM4SF1 influences the activity of proteins such as AKT and ERK which are important in mediating responses to growth factor signals and cellular stress. Its interactions with integrins also modulate signaling and structural dynamics of the cellular environment.
- Associated diseases and disorders
TM4SF1 is linked to several cancers including breast and lung cancer. Its elevated expression levels contribute to tumor growth and metastasis through enhancement of cell migration and angiogenesis. TM4SF1 is also associated with abnormal endothelial cell function influencing angiogenic diseases through its connections with proteins like VEGFR2 and integrin αVβ3. Understanding TM4SF1's role in these conditions can assist in developing therapeutic strategies.
Product promise
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4 product images
Sanger Sequencing - Human TM4SF1 (Transmembrane 4 L6 family member 1) knockout HeLa cell lysate (ab258232)
Allele-2: 1 bp insertion in exon 1
Sanger Sequencing - Human TM4SF1 (Transmembrane 4 L6 family member 1) knockout HeLa cell lysate (ab258232)
Allele-1: 0 bp insertion in exon 1
Sanger Sequencing - Human TM4SF1 (Transmembrane 4 L6 family member 1) knockout HeLa cell lysate (ab258232)
Allele-4:
Sanger Sequencing - Human TM4SF1 (Transmembrane 4 L6 family member 1) knockout HeLa cell lysate (ab258232)
Allele-3:
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