AB258233

Human TMED10 (TMP21) knockout HEK-293T cell lysate

Name: Human TMED10 (TMP21) knockout HEK-293T cell lysate (ab258233) | Abcam
Brand: Abcam Limited
SKU: AB258233
Price: 670 USD
Availability: InStock

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TMED10 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon1.

View Alternative Names

1110014C03Rik, 21 kDa transmembrane-trafficking protein, MGC102351, P23, S31I125, S31III125, TMED 10, TMEDA_HUMAN, Tmed10, Tmp-21-I, Transmembrane emp24 domain-containing protein 10, Transmembrane emp24-like trafficking protein 10 (yeast), Transmembrane protein Tmp21, Transmembrane trafficking protein 21kD, p24 family protein delta-1, p24(DELTA), p24delta, p24delta1

3 Images

Lab

Western blot - Human TMED10 (TMP21) knockout HEK-293T cell lysate (AB258233)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : TMED10 knockout HEK293T cell lysate (20 ug)
Lane 3 : U-87 MG cell lysate (20 ug)
Lane 4 : Raji cell lysate (20 ug)

ab134948 was shown to specifically react with TMP21 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266227 (knockout cell lysate ab258233) was used. Wild-type and TMP21 knockout samples were subjected to SDS-PAGE. ab134948 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TMP21 antibody [EPR9036(B)] (<a href='/en-us/products/primary-antibodies/tmp21-antibody-epr9036b-ab134948'>ab134948</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

TMED10 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human TMED10 (TMP21) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-tmed10-tmp21-knockout-hek-293t-cell-line-ab266227'>ab266227</a>)

Lane 3:

U-87 MG cell lysate at 20 µg

Lane 4:

Raji cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 25 kDa,9 kDa

Observed band size: 13 kDa,19 kDa

false

Lab

Western blot - Human TMED10 (TMP21) knockout HEK-293T cell lysate (AB258233)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : TMED10 knockout HEK293T cell lysate (20 ug)
Lane 3 : U-87 MG cell lysate (20 ug)
Lane 4 : Raji cell lysate (20 ug)

ab133771 was shown to specifically react with TMP21 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266227 (knockout cell lysate ab258233) was used. Wild-type and TMP21 knockout samples were subjected to SDS-PAGE. ab133771 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TMP21 antibody [EPR9037(B)] (<a href='/en-us/products/primary-antibodies/tmp21-antibody-epr9037b-ab133771'>ab133771</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

TMED10 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human TMED10 (TMP21) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-tmed10-tmp21-knockout-hek-293t-cell-line-ab266227'>ab266227</a>)

Lane 3:

U-87 MG cell lysate at 20 µg

Lane 4:

Raji cell lysate at 20 µg

Secondary

All lanes:

Predicted band size: 25 kDa,27 kDa,37 kDa,46 kDa

Observed band size: 19 kDa,27 kDa

false

Sanger Sequencing - Human TMED10 (TMP21) knockout HEK-293T cell lysate (AB258233)

Sanger seq

Unknown

Sanger Sequencing - Human TMED10 (TMP21) knockout HEK-293T cell lysate (AB258233)

Homozygous : 2 bp insertion in exon1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name

TMED10

Gene editing type

Knockout

Gene editing method

CRISPR technology

Knockout validation

Sanger Sequencing, Western blot

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMP21 also known as transmembrane protein 21 or by its alternate name p23 is a protein that plays a significant role in cellular functions. It has an approximate molecular mass of 21 kDa. TMP21 is mainly expressed in the brain but is also found in a variety of other tissues. Mechanically TMP21 functions as a part of the secretory pathway within the cell contributing to the processing and maturation of nascent proteins. It is known to regulate the trafficking of proteins across cellular compartments which is an important aspect of maintaining cellular homeostasis.

Biological function summary

TMP21 affects the modulation of the gamma-secretase complex which is essential in the generation of amyloid precursor protein (APP) and its subsequent processing. This protein acts as a negative regulator of gamma-secretase activity leading to alterations in APP cleavage. By being part of this complex TMP21 exerts influence over processes such as signal transduction and protein metabolism. Its expression levels and activity can impact neurophysiological functions due to its presence in nervous tissues.

Pathways

TMP21 integrates into the amyloidogenic pathway influencing amyloid-beta peptide production. This pathway is associated with the APP processing where TMP21 regulates the cleavage by controlling gamma-secretase activity. Another pathway involving TMP21 is the protein transport pathway facilitating the orderly movement of proteins through the early secretory pathway. Within these frameworks TMP21 interacts with proteins like presenilin which is a significant component of the gamma-secretase complex thereby affecting its overall functionality.

TMP21 has strong links to Alzheimer's disease and cancer. In Alzheimer's disease altered TMP21 function can lead to improper processing of APP resulting in increased amyloid-beta production and plaque formation. This relationship highlights TMP21's connection with presenilin which is important in amyloidogenic processes and influences disease progression. Additionally fluctuations in TMP21 expression and activity have been observed in certain cancers suggesting its role in aberrant cellular growth and migration due to its involvement in protein trafficking pathways. Understanding TMP21's interactions in these diseases can provide insights into potential therapeutic targets.

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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human TMED10 (TMP21) knockout HEK-293T cell lysate

Western blot - Human TMED10 (TMP21) knockout HEK-293T cell lysate (AB258233)

Western blot - Human TMED10 (TMP21) knockout HEK-293T cell lysate (AB258233)

Sanger Sequencing - Human TMED10 (TMP21) knockout HEK-293T cell lysate (AB258233)

Key facts

Cell type

Species or organism

Tissue

Knockout validation

Mutation description

Reactivity data

Product details

What's included?

Properties and storage information

Gene name

Gene editing type

Gene editing method

Knockout validation

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Quality control

STR analysis

Cell culture

Biosafety level

Adherent/suspension

Gender

Product protocols

Complementary Products

Primary Antibodies

Proteins & Peptides

Proteins & Peptides

Proteins & Peptides

Product promise