TMEM106B KO cell lysate available now. KO validated by Next Generation Sequencing. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift: 100%.
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Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Knockout validation
- Next Generation Sequencing
- Mutation description
- Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift: 100%
Alternative names
2310036D22Rik, 5830455K21Rik, 6430519M21Rik, AI428776, AI661344, FLJ11273, LRRGT00101, MGC33727, MGC94135, T106B_HUMAN, Tmem106b, Transmembrane protein 106B
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TMEM106B KO cell lysate available now. KO validated by Next Generation Sequencing. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift: 100%.
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Knockout validation
- Next Generation Sequencing
- Mutation description
- Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift: 100%
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- TMEM106B
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Next Generation Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TMEM106B also known as transmembrane protein 106B is a type II single-pass transmembrane protein with a molecular mass of approximately 33 kDa. It is expressed in specific regions of the brain as well as in other tissue types such as the lungs and immune system cells. Its expression levels are especially elevated in microglia and oligodendrocytes which are brain cells that play important roles in immune response and myelination respectively. Structurally TMEM106B contains a transmembrane domain and its precise biochemical function is still under investigation though it is thought to participate in lysosomal and vesicular trafficking.
- Biological function summary
TMEM106B is involved in several cellular processes impacting lysosome function and trafficking. It may become part of a protein complex that manages lysosomal positioning and pathway regulation within the cell. TMEM106B also affects cellular homeostasis and protein degradation which suggests a role in maintaining the proper balance of proteins and lipids within lysosomes. Alteration in TMEM106B expression or function can cause changes in lysosomal size and distribution indicating its role in cellular logistics.
- Pathways
The TMEM106B protein becomes involved in the endolysosomal pathway and the autophagy-lysosome pathway. These pathways are critical for processing and breakdown of cellular waste and recycling of cellular components. In these pathways TMEM106B interacts with other proteins such as SORT1 (sortilin 1) and GRN (progranulin) both of which have important roles in protein sorting and trafficking. By influencing these pathways TMEM106B helps maintain cellular health and function.
- Associated diseases and disorders
TMEM106B links to neurodegenerative conditions such as frontotemporal lobar degeneration and possibly Alzheimer's disease. Mutations or abnormal expression levels of TMEM106B have been implicated in the pathogenesis and progression of these diseases. In frontotemporal lobar degeneration TMEM106B interacts with proteins like GRN where changes can lead to neurodegeneration. In Alzheimer's disease abnormal lysosomal function tied to TMEM106B may contribute to the accumulation of pathogenic proteins.
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Next Generation Sequencing - Human TMEM106B knockout A549 cell lysate (ab273769)
Knockout achieved by CRISPR/Cas9; X = 1 bp insertion; Frameshift: 100%
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