Human TMEM106C knockout HEK-293T cell lysate
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TMEM106C KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 106 bp deletion in exon2 and 70 bp insertion in exon2 and Insertion of the selection cassette in exon2.
View Alternative Names
Endoplasmic reticulum membrane protein overexpressed in cancer, T106C_HUMAN, TMEM106C, Transmembrane protein 106C
- Sanger seq
Unknown
Sanger Sequencing - Human TMEM106C knockout HEK-293T cell lysate (AB263386)
Allele-3 : 106 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human TMEM106C knockout HEK-293T cell lysate (AB263386)
Allele-1 : 70 bp insertion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human TMEM106C knockout HEK-293T cell lysate (AB263386)
Allele-2 : Insertion of the selection cassette in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein can participate in processes related to cellular homeostasis and signaling pathways. Although it is not widely characterized as part of a larger complex the protein likely interacts with other membrane-associated proteins to mediate its effects. Potential biological activities may include regulatory mechanisms that ensure proper cellular function and communication.
Pathways
TMEM106C interacts with components involved in cellular signaling and communication pathways. It may be involved in pathways like the PI3K-Akt signaling pathway which is important for regulating cell cycle and survival. TMEM106C may also share relationships with proteins such as Akt and PI3K which are central to these pathways contributing to proliferative and survival signaling cascades.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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