Human TMEM126A knockout HeLa cell lysate
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TMEM126A KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and Insertion of the selection cassette in exon2.
View Alternative Names
OPA7, transmembrane protein 126A
- Sanger seq
Unknown
Sanger Sequencing - Human TMEM126A knockout HeLa cell lysate (AB263387)
Allele-1 : 1 bp insertion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human TMEM126A knockout HeLa cell lysate (AB263387)
Allele-2 : Insertion of the selection cassette in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TMEM126A contributes to the stability and assembly of the mitochondrial respiratory chain Complex I. It forms part of the mitochondrial contact site and cristae organizing system (MICOS) essential for maintaining the inner membrane architecture. By assisting in Complex I assembly TMEM126A supports efficient cellular respiration and energy production which is critical for cell survival and activity.
Pathways
The role of TMEM126A is significant in oxidative phosphorylation and mitochondrial respiration pathways. It interacts closely with Complex I components like NDUFAF1 and NDUFS1. These interactions are vital for proper electron transport chain function impacting cellular energy homeostasis and metabolic regulation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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