Human TMEM126B knockout HEK-293T cell lysate
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TMEM126B KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3.
View Alternative Names
HT007, MGC111203, Transmembrane protein 126B
- Sanger seq
Unknown
Sanger Sequencing - Human TMEM126B knockout HEK-293T cell lysate (AB259185)
Homozygous : 1 bp insertion in exon 3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein participates in the assembly of the mitochondrial membrane protein complex. It operates as a member of the mitochondrial complex I assembly (MCIA) complex which comprises several other assembly factors. These factors collaborate to promote efficient positioning and stabilization of complex I within the mitochondrial membrane. This process ensures adequate electron transport chain function which is vital for ATP production and overall energy metabolism.
Pathways
TMEM126B is essential for mitochondrial respiratory function specifically in the oxidative phosphorylation pathway. This pathway involves multiple steps for the efficient production of ATP. TMEM126B works alongside proteins such as NDUFAF1 and ECSIT facilitating complex assembly to smooth the transfer of electrons from NADH to ubiquinone. The protein contributes to the function of the electron transport chain and uncouples energy production from heat release impacting cellular energy efficiency.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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