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AB263389

Human TMEM140 knockout A549 cell lysate

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TMEM140 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 4 bp deletion in exon2 and 8 bp deletion in exon2.

View Alternative Names

FLJ11000, TM140_HUMAN, TMEM140, Transmembrane protein 140

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Sanger Sequencing - Human TMEM140 knockout A549 cell lysate (AB263389)
  • Sanger seq

Unknown

Sanger Sequencing - Human TMEM140 knockout A549 cell lysate (AB263389)

Allele-2 : 4 bp deletion in exon2

Sanger Sequencing - Human TMEM140 knockout A549 cell lysate (AB263389)
  • Sanger seq

Unknown

Sanger Sequencing - Human TMEM140 knockout A549 cell lysate (AB263389)

Allele-1 : 8 bp deletion in exon2

Sanger Sequencing - Human TMEM140 knockout A549 cell lysate (AB263389)
  • Sanger seq

Unknown

Sanger Sequencing - Human TMEM140 knockout A549 cell lysate (AB263389)

Allele-3 : 1 bp deletion in exon2

Key facts

Cell type

A549

Species or organism

Human

Tissue

Lung

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 4 bp deletion in exon2 and 8 bp deletion in exon2.

Disease

Carcinoma

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
TMEM140
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM140 also known as Transmembrane Protein 140 is a membrane protein with an approximate mass of 31 kDa. This protein spans the cell membrane and currently researchers identify its presence in various tissues including the brain and testis. TMEM140 involves in cellular signal transduction due to its integral membrane position and though the precise mechanical action remains under exploration experts suspect it plays a part in intracellular communication.
Biological function summary

TMEM140 impacts cell processes involving cellular differentiation and development. The protein does not traditionally belong to any larger multiprotein complex but preliminary evidence suggests it may interact with specific signaling molecules. Its expression and function imply potential roles in neurogenesis and gametogenesis contributing to the broader understanding of development.

Pathways

TMEM140 connects with pathways involving neural development and reproductive processes. It appears to interact with proteins related to these pathways which include other transmembrane proteins integral to cellular signaling. Experts study its involvement within MAPK signaling cascades a pathway important for transmitting extracellular signals to the cell nucleus affecting processes like growth and differentiation.

Alterations in TMEM140 function link to neurodegenerative disorders like Alzheimer's disease and certain fertility issues. The protein interacts with proteins such as tau in neuronal contexts which relates to neurodegeneration. In reproductive health aberrant TMEM140 expression might affect proteins in the spermatogenesis pathway potentially leading to infertility.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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Product promise

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