Human TMEM14B knockout HEK-293T cell lysate
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TMEM14B KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 19 bp deletion in exon 2.
View Alternative Names
FLJ60468, MGC1223, OTTHUMP00000214289, OTTHUMP00000214290, OTTHUMP00000214297, OTTHUMP00000214298, OTTHUMP00000214299, RP3-417M14.1, TM14B_HUMAN, Transmembrane protein 14B
- Sanger seq
Unknown
Sanger Sequencing - Human TMEM14B knockout HEK-293T cell lysate (AB259187)
Homozygous : 19 bp deletion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TMEM14B is key for mitochondrial biogenesis and the regulation of cellular energy metabolism. It is an integral component of the mitochondrial complex and contributes to the proper assembly of respiratory chain complexes. This protein facilitates the import of other essential proteins into the mitochondrial matrix ensuring efficient mitochondrial function and cellular energy homeostasis. Its activity is closely associated with energy production and apoptosis pathways.
Pathways
TMEM14B has essential roles in the oxidative phosphorylation and apoptosis pathways. These pathways are vital for energy production and programmed cell death respectively. TMEM14B's interaction with cytochrome c oxidase and caspases highlights its involvement in mitochondrial respiration and apoptotic regulation. Through these pathways TMEM14B collaborates with other mitochondrial proteins such as ATP synthase to support cellular energetic processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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