TMEM14B KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 19 bp deletion in exon 2.
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Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 19 bp deletion in exon 2.
Alternative names
FLJ60468, MGC1223, OTTHUMP00000214289, OTTHUMP00000214290, OTTHUMP00000214297, OTTHUMP00000214298, OTTHUMP00000214299, RP3-417M14.1, TM14B_HUMAN, Transmembrane protein 14B
What's included?
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TMEM14B KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 19 bp deletion in exon 2.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 19 bp deletion in exon 2.
- Concentration
Properties
- Gene name
- TMEM14B
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TMEM14B also known as Transmembrane Protein 14B is a mitochondrial inner membrane protein with a molecular weight of approximately 15 kDa. It is involved in the transport of molecules across the mitochondrial membranes. TMEM14B is expressed in a variety of tissues with a significant presence in the respiratory and nervous systems. The protein comprises several transmembrane helices playing an important role in maintaining mitochondrial function.
- Biological function summary
TMEM14B is key for mitochondrial biogenesis and the regulation of cellular energy metabolism. It is an integral component of the mitochondrial complex and contributes to the proper assembly of respiratory chain complexes. This protein facilitates the import of other essential proteins into the mitochondrial matrix ensuring efficient mitochondrial function and cellular energy homeostasis. Its activity is closely associated with energy production and apoptosis pathways.
- Pathways
TMEM14B has essential roles in the oxidative phosphorylation and apoptosis pathways. These pathways are vital for energy production and programmed cell death respectively. TMEM14B's interaction with cytochrome c oxidase and caspases highlights its involvement in mitochondrial respiration and apoptotic regulation. Through these pathways TMEM14B collaborates with other mitochondrial proteins such as ATP synthase to support cellular energetic processes.
- Associated diseases and disorders
TMEM14B's dysfunction is linked to neurodegenerative disorders and metabolic syndromes. Its improper functioning may lead to impaired mitochondrial activity contributing to conditions like Alzheimer's disease and metabolic syndrome. The protein relation with cytochrome c in these disorders highlights TMEM14B's involvement in neuronal cell death and metabolic dysregulation. Understanding TMEM14B's role in these diseases can provide insights into potential therapeutic targets.
Product promise
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1 product image
Sanger Sequencing - Human TMEM14B knockout HEK-293T cell lysate (ab259187)
Homozygous: 19 bp deletion in exon 2
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