STING1 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 insertion Frameshift: 99.58%.
Images
Key facts
- Cell type
THP-1
- Species or organism
Human
- Tissue
Blood
- Knockout validation
Next Generation Sequencing, Western blot
- Mutation description
Knockout achieved by CRISPR/Cas9 X = 1 insertion Frameshift: 99.58%
Alternative names
ERIS, Endoplasmic reticulum interferon stimulator, FLJ38577, MITA, MPYS, Mediator of IRF3 activation, Mitochondrial mediator of IRF3 activation, N terminal methionine proline tyrosine serine plasma membrane tetraspanner, NET23, Stimulator of interferon genes, Stimulator of interferon genes protein, TM173_HUMAN, Tmem173, Transmembrane protein 173, endoplasmic reticulum IFN stimulator, hMITA, hSTING
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STING1 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 insertion Frameshift: 99.58%.
Key facts
- Cell type
THP-1
- Species or organism
Human
- Tissue
Blood
- Knockout validation
Next Generation Sequencing, Western blot
- Mutation description
Knockout achieved by CRISPR/Cas9 X = 1 insertion Frameshift: 99.58%
- Disease
Acute Monocytic Leukemia
- Concentration
Properties
- Gene name
STING1
- Gene editing type
Knockout
- Gene editing method
CRISPR technology
- Knockout validation
Next Generation Sequencing, Western blot
Quality control
- STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
EU: 1 US: 1
- Adherent/suspension
Suspension
- Gender
Male
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
-20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The stimulator of interferon genes (STING) also known as TMEM173 or MPYS is a critical transmembrane protein with a molecular weight of approximately 42 kDa. It is primarily expressed in the endoplasmic reticulum of various cell types including immune cells where it plays a central role in sensing cytosolic DNA. STING binds to cyclic dinucleotides produced by the enzyme cGAS upon recognition of aberrant DNA in the cytosol. This binding initiates activation and translocation of STING to the Golgi apparatus facilitating further signaling events.
- Biological function summary
STING serves as a pivotal regulator in the innate immune response to viral and bacterial infections. It operates by forming a signaling complex with kinases and other effector proteins which subsequently leads to the activation of transcription factors such as IRF3 and NF-kB. These transcription factors then induce the expression of type I interferons and other cytokines important for mounting an effective antiviral response. The STING pathway therefore enhances the immune system's ability to detect and respond to pathogens.
- Pathways
The activity of STING is integral to the cGAS-STING pathway a significant cytosolic DNA-sensing pathway involved in innate immunity. Upon activation STING interacts with TBK1 a kinase that further phosphorylates IRF3 promoting its nuclear translocation and activation. Beyond this STING also intersects with pathways involving autophagy a cellular process necessary for clearing pathogens and damaged cellular components. Through these pathways STING critically contributes to upholding cellular homeostasis and immune defense.
- Associated diseases and disorders
The dysregulation of STING is linked to autoinflammatory diseases and certain cancers. Abnormal STING activation can lead to chronic inflammation a feature observed in diseases such as STING-associated vasculopathy with onset in infancy (SAVI). STING's role in cancer is also notable where its ability to activate immune cells can be harnessed in immunotherapy yet its chronic activation may promote tumorigenesis. In cancer STING often interacts with proteins like K-Ras influencing tumor growth and response to therapies.
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4 product images
Western blot - Human TMEM173 knockout THP-1 cell lysate (ab270516)
Lane 1: Wild-type THP-1 cell lysate 20 μg
Lane 2: TMEM173 knockout THP-1 cell lysate 20 μg
Lane 3: Human Tonsil cell lysate 20 μg
Lane 4: Human Thymus cell lysate 20 μg
False colour image of Western blot: Anti-STING antibody [EPR13130] staining at 1/1000 dilution, shown in green; loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) staining at 1/20000 dilution, shown in red. In Western blot, Anti-STING antibody [EPR13130] ab181125 was shown to bind specifically to STING. A band was observed at 37 kDa in wild-type THP-1 cell lysates with no signal observed at this size in TMEM173 knockout cell line Human TMEM173 knockout THP-1 cell line ab270493 (knockout cell lysate ab270516). To generate this image, wild-type and TMEM173 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.All lanes: Western blot - Anti-STING antibody [EPR13130] (Anti-STING antibody [EPR13130] ab181125) at 1/1000 dilution
Lane 1: Wild-type THP-1 cell lysate at 20 µg
Lane 2: TMEM173 knockout THP-1 cell lysate at 20 µg
Lane 3: Human Tonsil cell lysate at 20 µg
Lane 4: Human Thymus cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 37 kDa
Western blot - Human TMEM173 knockout THP-1 cell lysate (ab270516)
Lane 1: Wild-type THP-1 cell lysate 20 μg
Lane 2: TMEM173 knockout THP-1 cell lysate 20 μg
Lane 3: Human Tonsil tissue lysate 20 μg
Lane 4: Human Thymus tissue lysate 20 μgLanes 1 - 4: Merged signal (red and green). Green - Anti-STING antibody [EPR13130-55] ab239074 observed at 37 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
Anti-STING antibody [EPR13130-55] ab239074 was shown to react with TMEM173 in wild-type THP-1 cells in Western blot with loss of signal observed in TMEM173 knockout cell line Human TMEM173 knockout THP-1 cell line ab270493 (TMEM173 knockout cell lysae ab270516). Wild-type THP-1 and TMEM173 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with Anti-STING antibody [EPR13130-55] ab239074 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-STING antibody [EPR13130-55] (Anti-STING antibody [EPR13130-55] ab239074) at 1/1000 dilution
Lane 1: Wild-type THP-1 cell lysate at 20 µg
Lane 2: TMEM173 knockout THP-1 cell lysate at 20 µg
Lane 3: Human Tonsil tissue lysate at 20 µg
Lane 4: Human Thymus tissue lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 37 kDa
Western blot - Human TMEM173 knockout THP-1 cell lysate (ab270516)
Lane 1: Wild-type THP-1 cell lysate 20 μg
Lane 2: TMEM173 knockout THP-1 cell lysate 20 μg
Lane 3: Human Tonsil tissue lysate 20 μg
Lane 4: Human Thymus tissue lysate 20 μgLanes 1 - 4: Merged signal (red and green). Green - Anti-STING antibody [SP339] ab227705 observed at 37 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
Anti-STING antibody [SP339] ab227705 was shown to react with TMEM173 in wild-type THP-1 cells in Western blot with loss of signal observed in TMEM173 knockout cell line Human TMEM173 knockout THP-1 cell line ab270493 (TMEM173 knockout cell lysate ab270516). Wild-type THP-1 and TMEM173 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with Anti-STING antibody [SP339] ab227705 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 400 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-STING antibody [SP339] (Anti-STING antibody [SP339] ab227705) at 1/400 dilution
Lane 1: Wild-type THP-1 cell lysate at 20 µg
Lane 2: TMEM173 knockout THP-1 cell lysate at 20 µg
Lane 3: Human Tonsil tissue lysate at 20 µg
Lane 4: Human Thymus tissue lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 37 kDa
Next Generation Sequencing - Human TMEM173 knockout THP-1 cell lysate (ab270516)
Knockout achieved by CRISPR/Cas9; X = 1 bp insertion; Frameshift = 99.58%
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