Human TMEM208 knockout HEK-293T cell lysate
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TMEM208 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 41 bp insertion in exon1 and 7 bp deletion in exon1.
View Alternative Names
HSPC171, TM208_HUMAN, Transmembrane protein 208
- Sanger seq
Unknown
Sanger Sequencing - Human TMEM208 knockout HEK-293T cell lysate (AB263390)
Allele-3 : 1 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human TMEM208 knockout HEK-293T cell lysate (AB263390)
Allele-1 : 7 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human TMEM208 knockout HEK-293T cell lysate (AB263390)
Allele-2 : 41 bp insertion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein serves an essential function in the formation and maintenance of autophagic vesicles. It operates as a component of the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) participating in cargo sorting and transport. TMEM208's role in vesicle nucleation is important for autophagy a process where cells remove unnecessary or dysfunctional components maintaining cellular homeostasis. This action indirectly supports cellular adaptation to stress.
Pathways
TMEM208 integrates into intracellular trafficking and autophagy pathways working alongside proteins such as LC3 and ATG-related proteins. Through its involvement in vesicular transport TMEM208 collaborates in the lysosomal degradation pathway a process important for recycling cellular materials. TMEM208's interaction with LC3 connects it to the macroautophagy pathway allowing the cell to respond effectively to nutrient deprivation or other cellular stressors.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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