Human TMEM64 (TMM64) knockout A549 cell lysate
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TMEM64 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 2 bp deletion in exon1.
View Alternative Names
TMEM64, TMM64_HUMAN, Transmembrane protein 64
- Sanger seq
Unknown
Sanger Sequencing - Human TMEM64 (TMM64) knockout A549 cell lysate (AB259191)
Allele-1 : 2 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human TMEM64 (TMM64) knockout A549 cell lysate (AB259191)
Allele-2 : 1 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TMM64 interacts with molecular complexes that regulate lipid metabolism and energy homeostasis. Within cells this protein contributes to the regulation of cellular energy status by modulating interactions between lipid droplets and mitochondria. It influences lipid storage and usage by affecting enzymes involved in lipid biosynthesis pathways. This suggests its involvement in energy balance and metabolic processes.
Pathways
TMM64 has significant roles in the lipid metabolism and insulin signaling pathways. Within these pathways it acts in conjunction with proteins such as AMP-activated protein kinase (AMPK) and peroxisome proliferator-activated receptors (PPARs). In the lipid metabolism pathway TMM64 modulates the response to metabolic signals assisting in the adaptation of lipid storage and mobilization. It influences how cells react to insulin signals affecting glucose uptake and storage as lipids.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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