Human TMEM69 knockout HeLa cell lysate
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TMEM69 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3.
View Alternative Names
TMEM69, TMM69_HUMAN, Transmembrane protein 69
- Sanger seq
Unknown
Sanger Sequencing - Human TMEM69 knockout HeLa cell lysate (AB263393)
Homozygous : 1 bp deletion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The functions associated with TMEM69 contribute to cellular signaling processes and membrane trafficking. The protein does not form part of larger protein complexes itself but interacts with several membrane-associated proteins for diverse cellular roles. Its activity impacts cellular homeostasis influencing both signal transduction pathways and the maintenance of cellular machinery.
Pathways
TMEM69 impacts both the PI3K/AKT signaling and ERK/MAPK pathways influencing cell growth and survival. In the PI3K/AKT pathway it enhances kinase activity contributing to cell survival and proliferation. Within the ERK/MAPK pathway TMEM69 modulates protein interactions leading to changes in cell differentiation. Proteins like AKT and ERK1/2 interact closely with TMEM69 in these pathways highlighting its involvement in critical cellular processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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