Human TMOD3 (Tropomodulin 3) knockout HeLa cell lysate
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TMOD3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon2 and 8 bp deletion in exon2.
View Alternative Names
OTTHUMP00000162615, TMOD3_HUMAN, Tropomodulin 3 (ubiquitous), Tropomodulin 3 ubiquitous, Tropomodulin-3, U-Tmod, Ubiquitous tropomodulin
- Sanger seq
Unknown
Sanger Sequencing - Human TMOD3 (Tropomodulin 3) knockout HeLa cell lysate (AB258236)
Allele-1 : 8 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human TMOD3 (Tropomodulin 3) knockout HeLa cell lysate (AB258236)
Allele-2 : 2 bp insertion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Tropomodulin 3 interacts with components of the cytoskeleton to help maintain cell shape and stability. It often functions as part of a multi-protein complex with tropomyosin which stabilizes the actin filament and protects it from depolymerization. Through its interaction with actin and tropomyosin TMOD3 influences cell motility division and muscle contraction essential processes in cellular and tissue dynamics.
Pathways
Tropomodulin 3 plays a part in the actin cytoskeleton regulation pathway. It regulates the turnover and length of actin filaments which connect to processes like cell adhesion and movement. The regulation of actin filament dynamics by TMOD3 influences interactions with associated proteins such as myosin which drives cellular movements and mechanical support.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB249317
Anti-Tropomodulin 3 antibody [EPR10303(B)] - BSA and Azide free
RabMAb|Recombinant
![Western blot - Anti-Tropomodulin 3 antibody [EPR10303(B)] - BSA and Azide free (AB249317)](https://content.abcam.com/products/images/tropomodulin-3-antibody-epr10303b-bsa-and-azide-free-ab249317--western-blot-img105018.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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