TMSB10 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon2.
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Key facts
- Cell type
- HCT116
- Species or organism
- Human
- Tissue
- Colon
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon2.
Alternative names
MIG 12, Migration inducing gene 12, Migration inducing protein 12, Ptmb10, TB 10, TYB10_HUMAN, Thyb 10, Thymosin beta-10, Tmsb10
What's included?
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TMSB10 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon2.
Key facts
- Cell type
- HCT116
- Species or organism
- Human
- Tissue
- Colon
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon2.
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- TMSB10
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Thymosin beta 10 also known as TMSB10 is a small protein that plays a role in the dynamics of the cytoskeleton. It has a mass of approximately 5 kDa. TMSB10 is found in various tissues with high expression levels in the brain kidney and heart. Its function involves binding to actin monomers preventing polymerization and consequently regulating actin filament assembly in cells.
- Biological function summary
TMSB10 significantly influences cell movement and proliferation. It serves as an actin-sequestering protein and is not generally part of a larger protein complex. TMSB10 promotes cell motility and plays an essential part in processes like wound healing and angiogenesis. Through these actions it helps in tissue regeneration and repair influencing the cell's structural organization and signaling.
- Pathways
TMSB10 is involved in the actin cytoskeleton pathway and cellular signaling pathways that govern movement and shape changes in cells. Its interaction with actin places it in relation to actin-related protein (ARP) complexes such as Arp2/3 which facilitate actin filament branching. In addition TMSB10's regulation of actin dynamics links it to pathways involving proteins like cofilin which modulates actin filament turnover.
- Associated diseases and disorders
TMSB10 shows a connection to cancer and cardiovascular disorders. Elevated levels of TMSB10 have been associated with tumor progression due to its role in enhancing cell movement and metastasis. In cardiovascular disorders changes in TMSB10 expression can impact cardiac function due to its influence on actin dynamics. Proteins such as vascular endothelial growth factor (VEGF) interact with TMSB10 during pathological conditions further illustrating its role in disease mechanisms.
Product promise
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1 product image
Sanger Sequencing - Human TMSB10 (Thymosin beta 10) knockout HCT116 cell lysate (ab259194)
Homozygous: 1 bp deletion in exon2
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