Human TMSB4X (Thymosin beta 4) knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human TMSB4X (Thymosin beta 4) knockout HEK-293T cell lysate (AB258725)
Allele-1 : 7 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human TMSB4X (Thymosin beta 4) knockout HEK-293T cell lysate (AB258725)
Allele-2 : 1 bp insertion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human TMSB4X (Thymosin beta 4) knockout HEK-293T cell lysate (AB258725)
Allele-3 : Insertion of the selection cassette in exon2
Complementary Products
Primary Antibodies
AB180191
Anti-USP9x antibody [EPR13809(B)] - N-terminal
RabMAb|Recombinant|KO Validated
![Western blot - Anti-USP9x antibody [EPR13809(B)] - N-terminal (AB180191)](https://content.abcam.com/products/images/usp9x-antibody-epr13809b-n-terminal-ab180191--western-blot-img18135.jpg)
- 5
- 1
![Western blot - Anti-FXYD3 antibody [EPR17160] (AB205534)](https://content.abcam.com/products/images/fxyd3-antibody-epr17160-ab205534--western-blot-img158045.jpg)
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Thymosin beta 4 influences several cellular processes including cell migration proliferation and differentiation. It is not part of a large complex but directly interacts with actin to modulate its behavior. Through its regulation of actin filament formation it plays a significant role in wound healing tissue regeneration and inflammatory responses. Furthermore it contributes to the migration of endothelial cells highlighting its importance in angiogenesis.
Pathways
Thymosin beta 4 contributes significantly to cell motility and the vascular endothelial growth factor (VEGF) pathway. It interacts with the protein profilin in the regulation of actin dynamics impacting cellular processes like cell movement and angiogenesis. By influencing these pathways Thymosin beta 4 aids in the body's repair mechanisms providing cellular structural support under varying physiological conditions.
Quality control
STR analysis
D5S818, TH01, D16S539, TPOX, CSF1PO, D13S317, D7S820
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com