Human TMUB1 knockout HeLa cell lysate
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TMUB1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 1 bp insertion in exon2.
View Alternative Names
C7orf21, Chromosome 7 Open Reading Frame 21, DULP, Dendritic Cell-Derived Ubiquitin-Like Protein, HOPS, Hepatocyte odd protein shuttling protein, SB144, TMUB1_HUMAN, Transmembrane and ubiquitin-like domain-containing protein 1, UNQ763/PRO1555, Ubiquitin-like protein DULP, Ubiquitin-like protein SB144
- WB
Lab
Western blot - Human TMUB1 knockout HeLa cell lysate (AB258237)
Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate (20µg)
Lane 2 : TMUB1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate (20µg)
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate cell lysate (20µg)
Lanes 1- 3 : Merged signal (red and green). Green - ab180586 observed at 27 kDa. Red - loading control ab8245 observed at 37 kDa.
ab180586 Recombinant Anti-TMUB1 antibody [EPR14066] was shown to specifically react with TMUB1 in wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) cells in western blot. Loss of signal was observed when knockout cell line ab265852 (knockout cell lysate ab258237) was used. Wild-type and TMUB1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab180586 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-TMUB1 antibody [EPR14066] (<a href='/en-us/products/primary-antibodies/tmub1-antibody-epr14066-ab180586'>ab180586</a>) at 1/10000 dilution
Lane 1:
Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg
Lane 2:
TMUB1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg
Lane 2:
Western blot - Human TMUB1 partial knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-tmub1-partial-knockout-hela-cell-line-ab265852'>ab265852</a>)
Lane 3:
HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate cell lysate at 20 µg
Predicted band size: 26 kDa
Observed band size: 27 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human TMUB1 knockout HeLa cell lysate (AB258237)
Allele-1 : 1 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human TMUB1 knockout HeLa cell lysate (AB258237)
Allele-2 : 1 bp insertion in exon2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
Knockout profile: Only the long form of the protein (lHOPS, 27 kDa) has been knocked-out from the parental cell line. The band observed in the KO lysate at 21 kDa is likely to represent a short form of the protein (sHOPS) (doi: 10.4161/cc.27054). We have not investigated the function of the remaining form of the protein.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
D16S539, TPOX, CSF1PO, D13S317, D7S820, D5S818, TH01
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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