Human TMUB1 knockout HeLa cell lysate

• WB

Lab

Western blot - Human TMUB1 knockout HeLa cell lysate (AB278185)

Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : TMUB1 knockout HeLa cell lysate 20 μg
Lane 3 : HepG2 cell lysate 20 μg
Lane 4 : Human Cerebellum tissue lysate 20 μg
Lanes 1 - 4 : Merged signal (red and green). Green - ab180586 observed at 27 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab180586 was shown to react with TMUB1 in wild-type HeLa cells in Western blot with loss of signal observed in TMUB1 knockout cell line ab278130 (knockout cell lysate ab278185). Wild-type HeLa and TMUB1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab180586 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 ^°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-TMUB1 antibody [EPR14066] (<a href='/en-us/products/primary-antibodies/tmub1-antibody-epr14066-ab180586'>ab180586</a>) at 1/10000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

TMUB1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human TMUB1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-tmub1-knockout-hela-cell-line-ab278130'>ab278130</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

Human Cerebellum tissue lysate at 20 µg

Predicted band size: 26 kDa

Observed band size: 27 kDa

false

• Sanger seq

Lab

Sanger Sequencing - Human TMUB1 knockout HeLa cell lysate (AB278185)

Allele-1 : 1 bp deletion and 159 bp deletion in exon 2