Human TNC (Tenascin C) knockout HeLa cell lysate
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TNC KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4 and Insertion of the selection cassette in exon4.
View Alternative Names
GP 150-225, TN-C, HXB, Tenascin, TNC, GMEM, Cytotactin, Glioma-associated-extracellular matrix antigen, Hexabrachion, JI, Neuronectin, Tenascin-C, TN, Myotendinous antigen
- Sanger seq
Unknown
Sanger Sequencing - Human TNC (Tenascin C) knockout HeLa cell lysate (AB257292)
Allele-1 : 1 bp deletion in exon4
- Sanger seq
Unknown
Sanger Sequencing - Human TNC (Tenascin C) knockout HeLa cell lysate (AB257292)
Allele-2 : Insertion of the selection cassette in exon4
Complementary Products
Primary Antibodies
AB108930
Anti-Tenascin C antibody [EPR4219]
RabMAb|Recombinant|KO Validated|20ul selling size
![Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EPR4219] (AB108930)](https://content.abcam.com/products/images/tenascin-c-antibody-epr4219-ab108930--immunohistochemistry-frozen-sections-img110582.jpg)
- 4
- 17
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The large hexamer known as Tenascin C plays a significant role in modulating cell adhesion migration and proliferation especially during developmental processes and injury responses. It incorporates into the existing extracellular matrix as a loose network which provides elasticity and supports cellular activities. This feature allows Tenascin C to interact with other matrix molecules and cell surface receptors to regulate tissue morphogenesis.
Pathways
Tenascin C actively participates in the Wnt and TGF-beta signaling pathways important for controlling cellular behavior in development and regeneration. It associates with integrins and glycoproteins to influence cell responses to signaling events in these pathways. By modulating these interactions Tenascin C can impact processes such as epithelial-to-mesenchymal transition and angiogenesis affecting tissue architecture and function.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com