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TNF KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 52 bp deletion in exon 4.

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Images

Western blot - Human TNF knockout THP-1 cell lysate (AB275507), expandable thumbnail
  • Western blot - Human TNF knockout THP-1 cell lysate (AB275507), expandable thumbnail
  • Western blot - Human TNF knockout THP-1 cell lysate (AB275507), expandable thumbnail
  • Sanger Sequencing - Human TNF knockout THP-1 cell lysate (AB275507), expandable thumbnail

Key facts

Cell type
THP-1
Species or organism
Human
Tissue
Blood
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 52 bp deletion in exon 4

Alternative names

APC1 protein, Cachectin, DIF, Differentiation inducing factor, Macrophage cytotoxic factor, TNF superfamily member 2, TNF, macrophage derived, TNF, monocyte derived, TNF-alpha, TNFA_HUMAN, TNFSF2, Tnf, Tumor Necrosis Factor, Membrane Form, Tumor necrosis factor, Tumor necrosis factor (TNF superfamily member 2), Tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, Tumor necrosis factor, soluble form

What's included?

1 Kit
Components
Human TNF knockout THP-1 cell lysate - Brefeldin A treated
1 x 100 µg
Human TNF knockout THP-1 cell lysate - LPS + Brefeldin A treated
1 x 100 µg
Human wild-type THP-1 cell lysate - Brefeldin A treated
1 x 100 µg
Human wild-type THP-1 cell lysate - LPS + Brefeldin A treated
1 x 100 µg

Recommended products

TNF KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 52 bp deletion in exon 4.

Key facts

Cell type
THP-1
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 52 bp deletion in exon 4
Disease
Acute Monocytic Leukemia
Concentration
Loading...

Properties

Gene name
TNF
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 1 US: 1
Adherent/suspension
Suspension
Gender
Male

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Notes


Knockout cell lysate achieved by CRISPR/Cas9.

Treatments:
Human TNF knockout THP-1 cell lysate - Brefeldin A (5 μg/ml, 6h)
Human wild-type THP-1 cell lysate - Brefeldin A (5 μg/ml, 6h)
Human TNF knockout THP-1 cell lysate - LPS (1 μg/ml, 16h) and Brefeldin A (5 μg/ml, during the last 6h of LPS treatment)
Human wild-type THP-1 cell lysate - LPS (1 μg/ml, 16h) and Brefeldin A (5 μg/ml, during the last 6h of LPS treatment)

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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