Human TNFRSF10A (DR4) knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
TNFRSF10A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 37 bp deletion in exon 2.
View Alternative Names
Apo2, CD261, Cytotoxic TRAIL receptor, DR4, Death receptor 4, NF related apoptosis-inducing ligand receptor 1, TNF receptor superfamily member 10a, TNF-related apoptosis-inducing ligand receptor 1, TNFRSF10A, TR10A_HUMAN, TRAIL receptor 1, TRAIL-R1, Tumor necrosis factor receptor superfamily member 10A, Tumor necrosis factor receptor superfamily member 10a variant 2
- Sanger seq
Unknown
Sanger Sequencing - Human TNFRSF10A (DR4) knockout HeLa cell lysate (AB258726)
Homozygous : 37 bp deletion in exon 2
- WB
Supplier Data
Western blot - Human TNFRSF10A (DR4) knockout HeLa cell lysate (AB258726)
Blocking and diluting buffer and concentration : 5% NFDM/TBST Performed under reducing conditions. In Western blot, ab312848 was shown to bind specifically to TNFRSF10A (DR4). A band was observed at 45 kDa in wild-type Hela cell lysates whereas loss of signal was observed in the TNFRSF10A (DR4) knockout cell line ab264687 (knockout cell lysate ab258726). In Western blot, anti- Vinculin antibody (ab129002) loading control staining at 1/10000 dilution. Exposure time : 15 seconds
All lanes:
Western blot - Anti-DR4 antibody [EPR28152-57] (<a href='/en-us/products/primary-antibodies/dr4-antibody-epr28152-57-ab312848'>ab312848</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 50 µg
Lane 2:
Western blot - Human TNFRSF10A (DR4) knockout HeLa cell lysate (ab258726) at 50 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 45 kDa
false
Exposure time: 15s
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The death receptor 4 plays an important role in mediating cell apoptosis. Upon ligand binding DR4 interacts with the adapter molecule FADD (FAS-associated protein with death domain) facilitating the formation of the death-inducing signaling complex (DISC). This complex recruits and activates caspase-8 which eventually leads to the activation of the downstream effector caspases driving the cell towards apoptosis. DR4 operates as a significant component of the extrinsic pathway of apoptosis a process important for maintaining cellular homeostasis and eliminating harmful cells.
Pathways
DR4 functions critically within the extrinsic apoptotic pathway. This pathway involves other death receptors such as DR5 which also binds TRAIL similarly leading to apoptosis through DISC formation and caspase activation. The extrinsic pathway interconnects with the intrinsic pathway via the mitochondria with proteins like Bid acting as a bridge between them. The engagement of these pathways highlights DR4’s importance in regulatory mechanisms of cell death tightly controlling cellular proliferation and survival.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com