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AB259196

Human TNKS1BP1 (Tankyrase binding protein 1) knockout HeLa cell lysate

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Human TNKS1BP1 (Tankyrase binding protein 1) knockout HeLa cell lysate. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

View Alternative Names

182 kDa tankyrase-1-binding protein, FLJ45975, KIAA1741, TAB182, TB182_HUMAN, Tankyrase 1 binding protein 1, Tankyrase 1 binding protein 1 182 kDa, Tankyrase 1 binding protein of 182 kDa, Tnks1bp1

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Sanger Sequencing - Human TNKS1BP1 (Tankyrase binding protein 1) knockout HeLa cell lysate (AB259196)
  • Sanger seq

Unknown

Sanger Sequencing - Human TNKS1BP1 (Tankyrase binding protein 1) knockout HeLa cell lysate (AB259196)

Homozygous : 10 bp deletion in exon2

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon2.

Disease

Adenocarcinoma

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
TNKS1BP1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tankyrase binding protein 1 also known as TAB182 or TNKS1BP1 interacts directly with tankyrase enzymes. It has a molecular mass of approximately 182 kDa. The protein mainly localizes in the cytoplasm and nucleus. Its interaction with tankyrase a poly(ADP-ribose) polymerase modulates various cell processes. This binding suggests TAB182 has important roles in managing protein stability and cellular localization reactions.
Biological function summary

TAB182 participates heavily in cellular regulation activities. It forms complexes with tankyrase contributing to the control of telomere length and maintenance. By engaging with numerous other proteins TAB182 influences functions like Wnt signaling and Axin degradation. It interacts with tankyrase to impact cellular structures like centrosomes hinting at a broad regulatory role in cell cycle regulation.

Pathways

TAB182's interaction with tankyrase places it within notable cellular pathways. It plays a role in the Wnt/β-catenin signaling pathway which regulates gene transcription and cellular differentiation. In this pathway TAB182 works closely with Axin and the destruction complex. Furthermore TAB182 is linked to the telomere elongation pathway collaborating with TRF1 to regulate telomere maintenance inheriting tankyrase association roles.

TAB182 connection implicates it in cancer and metabolic disorders. In cancer TAB182 dysregulation might alter telomere maintenance facilitating oncogenesis in conjunction with proteins like TRF1. Regarding metabolic disorders disruptions in Wnt signaling pathways involving TAB182 and Axin could contribute to obesity or type 2 diabetes as these pathways regulate adipogenesis and insulin sensitivity. Understanding TAB182's role helps uncover potential therapeutic targets for these diseases.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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