JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB257286

Human TOP2B (Topoisomerase II beta) knockout HEK-293T cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

TOP2B KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 3 and 17 bp deletion in exon 3.

View Alternative Names

Antigen MLAA 44, DNA topoisomerase 2-beta, DNA topoisomerase II, DNA topoisomerase II 180 kD, DNA topoisomerase II beta, DNA topoisomerase II beta isozyme, TOP IIB, TOP2B_HUMAN, Top 2, Top2 beta, Topo II beta, Topoisomerase (DNA) II beta, Topoisomerase (DNA) II beta 180kDa, Topoisomerase IIb, U937 associated antigen, beta isozyme

3 Images
Western blot - Human TOP2B (Topoisomerase II beta) knockout HEK-293T cell lysate (AB257286)
  • WB

Lab

Western blot - Human TOP2B (Topoisomerase II beta) knockout HEK-293T cell lysate (AB257286)

Lane 1 : Wild-type HEK-293T cell lysate (40µg)

Lane 2 : TOP2B knockout HEK-293T cell lysate (40µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab125297 observed at 183 kDa. Red - loading control ab7291 observed at 50 kDa.

ab125297 Anti-Topoisomerase II beta/TOP2B antibody was shown to specifically react with Topoisomerase II beta/TOP2B in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266340 (knockout cell lysate ab257286) was used. Wild-type and Topoisomerase II beta/TOP2B knockout samples were subjected to SDS-PAGE. ab125297 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Topoisomerase II beta/TOP2B antibody (<a href='/en-us/products/primary-antibodies/topoisomerase-ii-beta-top2b-antibody-ab125297'>ab125297</a>) at 1 µg/mL

Lane 1:

Wild-type HEK-293T cell lysate at 40 µg

Lane 1:

Western blot - Human TOP2B (Topoisomerase II beta) knockout HEK-293T cell lysate (ab257286)

Lane 1:

Western blot - Human TOP2B (Topoisomerase II beta) knockout HEK-293T cell pellet (<a href='/en-us/products/cell-lysates/human-top2b-topoisomerase-ii-beta-knockout-hek-293t-cell-pellet-ab279120'>ab279120</a>)

Lane 2:

TOP2B knockout HEK-293T cell lysate at 40 µg

Lane 2:

Western blot - Human TOP2B (Topoisomerase II beta) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-top2b-topoisomerase-ii-beta-knockout-hek-293t-cell-line-ab266340'>ab266340</a>)

Predicted band size: 183 kDa

Observed band size: 183 kDa

false

Sanger Sequencing - Human TOP2B (Topoisomerase II beta) knockout HEK-293T cell lysate (AB257286)
  • Sanger seq

Unknown

Sanger Sequencing - Human TOP2B (Topoisomerase II beta) knockout HEK-293T cell lysate (AB257286)

Allele-2 : 13 bp deletion in exon 3

Sanger Sequencing - Human TOP2B (Topoisomerase II beta) knockout HEK-293T cell lysate (AB257286)
  • Sanger seq

Unknown

Sanger Sequencing - Human TOP2B (Topoisomerase II beta) knockout HEK-293T cell lysate (AB257286)

Allele-1 : 17 bp deletion in exon 3

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 3 and 17 bp deletion in exon 3.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257286-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human TOP2B knockout HEK293T cell lysate", "number":"AB257286-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB257286-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
TOP2B
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Topoisomerase II beta (TOP2B) is an essential enzyme that alters the topological states of DNA during transcription replication and chromatin remodeling. It accomplishes this by inducing transient double-strand breaks in the DNA helix to facilitate the passage of another DNA segment through this break before the enzyme reseals the break. TOP2B is also known as DNA topoisomerase (ATP-hydrolyzing) and has a molecular mass of approximately 180 kDa. The enzyme is widely expressed across various tissues but the highest levels are found in the brain and testis reflecting its vital role in these organs.
Biological function summary

Topoisomerase II beta contributes to the regulation of genetic processes such as transcription chromosome segregation and DNA repair. It is not a lone player; TOP2B often functions within a complex involving other proteins that assist in stabilizing the DNA topology changes it mediates. The enzyme's activity ensures proper chromatin dynamics particularly in neurons where it influences long-range transcription regulation essential for neural development and function.

Pathways

Topoisomerase II beta plays a significant role in DNA damage response and repair pathways interacting with intricate network proteins like RPA (Replication Protein A) and BRCA1. In addition TOP2B is involved in the transcriptional regulation pathway where it facilitates changes in chromatin structure necessary for the transcription machinery to access gene sequences. Its interactions with these proteins highlight its integration in cellular mechanisms maintaining genomic stability and regulating gene expression.

Topoisomerase II beta has associations with cancer and neurodegenerative diseases. Aberrations in TOP2B activity can lead to genomic instability contributing to oncogenesis particularly in types of leukemia and solid tumors. Elevated or dysfunctional activity of TOP2B is often observed alongside proteins like the tumor suppressor p53 an important regulator of cell cycle and apoptosis linking their combined malfunction to cancer progression. Additionally irregular TOP2B function is connected to Alzheimer's disease with evidence suggesting its involvement in neuronal death often alongside proteins like Tau further implicating its role in neurodegenerative pathologies.
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB109524

Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377]

RabMAb|Recombinant

Western blot - Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377] (AB109524)

  • 5
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com