Human TOR3A knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
TOR3A KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon1 and 1 bp deletion in exon1 and 1 bp insertion in exon1.
View Alternative Names
ADIR, ADIR2, ATP dependant interferon response protein 1, ATP dependant interferon responsive, ATP-dependent interferon-responsive protein, FLJ22345, MGC111104, TOR3A_HUMAN, Torsin family 3 member A, Torsin-3A
- Sanger seq
Unknown
Sanger Sequencing - Human TOR3A knockout HeLa cell lysate (AB263396)
Allele-2 : 1 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human TOR3A knockout HeLa cell lysate (AB263396)
Allele-1 : 10 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human TOR3A knockout HeLa cell lysate (AB263396)
Allele-3 : 1 bp insertion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein participates in processes related to maintaining cellular homeostasis. Although not a direct component of large protein complexes TOR3A interacts with molecular chaperones to assist in the correct folding and assembly of polypeptides. This function is foundational for maintaining cell survival and minimizing stress responses. The protein may interact with elements of the cell's quality control systems to facilitate the degradation of misfolded proteins.
Pathways
TOR3A takes part in cellular stress response and protein quality control pathways. It functions alongside other proteins like heat shock proteins (HSPs) to modulate the folding and repair of cellular proteins ensuring that they are correctly synthesized and functional. These pathways are key to cellular homeostasis helping cells to cope with stress and alterations in their environment.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com