Human TP53I3 (PIG3) knockout HeLa cell lysate
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TP53I3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3.
View Alternative Names
Putative quinone oxidoreductase, QORX_HUMAN, Quinone oxidoreductase PIG3, TP53I3, Tumor protein p53-inducible protein 3, p53-induced gene 3 protein, quinone oxidoreductase homolog
- Sanger seq
Unknown
Sanger Sequencing - Human TP53I3 (PIG3) knockout HeLa cell lysate (AB258243)
Homozygous : 1 bp deletion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PIG3 mediates cell apoptosis in response to DNA damage and oxidative stress. It forms part of a complex with p53 contributing to the regulation of apoptotic pathways. As an oxidoreductase PIG3 initiates pro-apoptotic changes within the cell. Its enzymatic activity involves producing reactive oxygen species (ROS) further amplifying the cell’s apoptotic initiations. Its role connects to cellular response mechanisms in stress conditions dealing with damaged or unhealthy cells.
Pathways
PIG3 operates within the important p53 apoptotic pathway and the DNA damage signaling pathway. It significantly interacts with p53 as PIG3 expression depends on p53 transcription factor activation. Also PIG3 connects with caspase pathways by influencing the downstream apoptosis cascade required for cell death. It acts as a link bridging initial DNA damage response and the execution of apoptosis providing a control node within these pathways.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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