Human TP53INP2 knockout HEK-293T cell lysate
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TP53INP2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4.
View Alternative Names
C20orf110, DOR, Diabetes and obesity regulated; p53 inducible protein U, PINH, T53I2_HUMAN, Tumor protein p53-inducible nuclear protein 2, p53-inducible protein U
- Sanger seq
Unknown
Sanger Sequencing - Human TP53INP2 knockout HEK-293T cell lysate (AB258733)
Homozygous : 1 bp insertion in exon4
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TP53INP2 plays a significant role in modulating the cellular stress response and apoptosis. It interacts with autophagy-related proteins to facilitate the degradation of damaged cellular components. TP53INP2 is not generally described as part of a larger protein complex but its interactions with several autophagy-related proteins underline its involvement in cellular cleaning and recycling systems aiding in the balance between cell survival and cell death.
Pathways
TP53INP2 connects to both the autophagy and apoptosis pathways. Within the autophagy pathway TP53INP2 collaborates with proteins like LC3 and Beclin-1 to promote the formation of autophagosomes. Meanwhile in apoptosis pathways TP53INP2 affects the activity of the tumor suppressor protein p53 providing a link between autophagic processes and programmed cell death especially under cellular stress conditions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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