Human TPD52L1 knockout HeLa cell lysate
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TPD52L1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
View Alternative Names
D53, D54, MGC73020, MGC8556, OTTHUMP00000017133, OTTHUMP00000017136, TPD53_HUMAN, Tumor protein D52 like 2, Tumor protein D52-like 1, Tumor protein D53, Tumor protein D54, hD53, hD54
- Sanger seq
Unknown
Sanger Sequencing - Human TPD52L1 knockout HeLa cell lysate (AB259198)
Allele-1 : 7 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human TPD52L1 knockout HeLa cell lysate (AB259198)
Allele-2 : Insertion of the selection cassette in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TPD52L1 influences cell proliferation and differentiation functions within the cell cycle. The protein does not form a complex but interacts with other proteins involved in regulating cell growth and signaling. For example it plays a role in vesicular trafficking and exocytosis which are important for maintaining cellular homeostasis. Therefore its function is important for both normal cellular functioning and response to changes in the cellular environment.
Pathways
TPD52L1 plays an active role in the PI3K/Akt signaling pathway which is critical for cell survival and proliferation. It also contributes to the ERK/MAPK pathway influencing gene transcription and cellular responses to growth signals. Within these pathways TPD52L1 interacts with proteins like Akt1 and MAPK which are pivotal in regulating cellular processes such as metabolism growth and apoptosis. Its role within these pathways highlights its involvement in important cellular decisions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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