Human TPGS2 (L17) knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
TPGS2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and Insertion of the selection cassette in exon1.
View Alternative Names
C18orf10, C18orf10 chromosome 18 open reading frame 10, Chromosome 18 open reading frame 10, DKFZP586M1523, HMFN0601, HsT3006, TPGS2_HUMAN, Tubulin polyglutamylase complex subunit 2
- Sanger seq
Unknown
Sanger Sequencing - Human TPGS2 (L17) knockout HeLa cell lysate (AB263397)
Allele-1 : 1 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human TPGS2 (L17) knockout HeLa cell lysate (AB263397)
Allele-2 : Insertion of the selection cassette in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The function of protein L17 involves participation in the formation of the ribosome complex. It interacts with other ribosomal proteins and rRNA molecules to ensure the accurate assembly of the ribosome. This assembly allows the ribosomal machinery to facilitate efficient translation of mRNA into polypeptides which are subsequently folded into functional proteins essential for cellular functions and proliferation.
Pathways
The protein L17 engages with translation-related pathways notably the ribosomal biogenesis pathway and the mTOR signaling pathway. The mTOR pathway regulates cell growth and metabolism in response to nutrients stress and growth factors. As part of the ribosome L17 indirectly associates with proteins like RPL5 and RPL11 essential for ribosome biogenesis and function forming a link between ribosome assembly and cellular regulation.
Quality control
STR analysis
D7S820, D5S818, TH01, D16S539, TPOX, CSF1PO, D13S317
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com