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AB258245

Human TPM3 (Tropomyosin 3) knockout HEK-293T cell lysate

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TPM3 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.

View Alternative Names

Alpha tropomyosin 3, Alpha tropomyosin slow skeletal, CFTD, Cytoskeletal tropomyosin TM30, FLJ41118, Gamma-tropomyosin, Heat stable cytoskeletal protein 30 kDa, MGC102590, MGC14582, MGC3261, MGC72094, NEM1, OK/SW-cl.5, OTTHUMP00000034019, OTTHUMP00000034171, OTTHUMP00000034172, TM-5, TM3, TM30, TM30nm, TPM3/NTRK1 FUSION GENE, INCLUDED, TPM3_HUMAN, TPMsk3, TRK, TRK ONCOGENE, INCLUDED, Tm5NM, Tpm 5, Trop 5, Tropomyosin 3 gamma, Tropomyosin alpha-3 chain, Tropomyosin gamma, Tropomyosin-3, Tropomyosin-5, gamma TM, hTM30nm, hTM5, hTMnm, hscp30

2 Images
Western blot - Human TPM3 (Tropomyosin 3) knockout HEK-293T cell lysate (AB258245)
  • WB

Lab

Western blot - Human TPM3 (Tropomyosin 3) knockout HEK-293T cell lysate (AB258245)

Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)
Lane 2 : TPM3 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)

ab113692 was shown to specifically react with Tropomyosin 3 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266422 (knockout cell lysate ab258245) was used. Wild-type and Tropomyosin 3 knockout samples were subjected to SDS-PAGE. ab113692 and Anti-beta Tubulin [EP1331Y] - Microtubule Marker (ab52901) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Tropomyosin 3 antibody [3D5AH3AB4] (<a href='/en-us/products/primary-antibodies/tropomyosin-3-antibody-3d5ah3ab4-ab113692'>ab113692</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

TPM3 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

Western blot - Human TPM3 (Tropomyosin 3) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-tpm3-tropomyosin-3-knockout-hek-293t-cell-line-ab266422'>ab266422</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-680rd-preadsorbed-ab216777'>ab216777</a>) at 1/10000 dilution

Predicted band size: 33 kDa

Observed band size: 37 kDa

false

Sanger Sequencing - Human TPM3 (Tropomyosin 3) knockout HEK-293T cell lysate (AB258245)
  • Sanger seq

Unknown

Sanger Sequencing - Human TPM3 (Tropomyosin 3) knockout HEK-293T cell lysate (AB258245)

Homozygous : 1 bp insertion in exon 2

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
TPM3
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tropomyosin 3 also known as TPM3 is an actin-binding protein that stabilizes actin filaments. This protein plays an important role in maintaining cytoskeleton integrity and regulating cell contraction. Tropomyosin 3 has a molecular weight of approximately 32.5 kDa and is widely expressed in various tissues especially in muscle cells and in some non-muscle tissues. This protein interacts with other proteins to form complexes that modulate the dynamics of the actin filament systems.
Biological function summary

Tropomyosin 3 modulates the access of other actin-binding proteins to the actin filament by covering and uncovering binding sites. It partners with actin and troponin complexes influencing muscle contraction and cell motility. Additionally TPM3 regulates the stability and flexibility of actin cortex important for cell shape and movement. In non-muscle cells it supports the formation of cell structures needed for signaling and transportation.

Pathways

Tropomyosin 3 functions in the regulation of the actin cytoskeleton pathway and the muscle contraction pathway. It coordinates with proteins like actin and myosin to facilitate these processes. In the actin cytoskeleton pathway TPM3 balances polymerization and depolymerization of actin important for maintaining cellular structures. The muscle contraction pathway relies on TPM3 to regulate calcium binding supporting muscular function and responsiveness.

Tropomyosin 3 mutations link to conditions like congenital myopathy and nemaline myopathy. Mutations in TPM3 affect muscle fiber regulation leading to muscle weakness and structural abnormalities. Congenital myopathy involves other proteins such as nebulin which cooperate with TPM3 in maintaining muscle architecture. In nemaline myopathy improper expression or structure of TPM3 contributes to defective sarcomere formation impairing muscle function.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Primary Antibodies

AB113692

Anti-Tropomyosin 3 antibody [3D5AH3AB4]

BOND RX™ Validated|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Tropomyosin 3 antibody [3D5AH3AB4] (AB113692)

  • 4
  • 1
Proteins & Peptides

AB119162

Recombinant Human Tropomyosin protein

SDS-PAGE - Recombinant Human Tropomyosin protein (AB119162)

  • 0
  • 0
Cell Lines & Lysates

AB266422

Human TPM3 (Tropomyosin 3) knockout HEK-293T cell line

Advanced Validation

Western blot - Human TPM3 (Tropomyosin 3) knockout HEK-293T cell line (AB266422)

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  • 0

Product promise

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