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AB257758

Human TPM4 knockout HEK-293T cell lysate

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TPM4 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and Insertion of the selection cassette in exon 1.

View Alternative Names

HEL-S-108, TM30p1, TMP 4, TPM4_HUMAN, Tropomyosin alpha-4 chain, Tropomyosin-4, epididymis secretory protein Li 108, wu:fb06e07, zgc:103436, zgc:63909

4 Images
Western blot - Human TPM4 knockout HEK-293T cell lysate (AB257758)
  • WB

Lab

Western blot - Human TPM4 knockout HEK-293T cell lysate (AB257758)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : TPM4 knockout HEK293T cell lysate (20 ug)
Lane 3 : HeLa cell lysate (20 ug)

ab181156 was shown to specifically react with TPM4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266606 (knockout cell lysate ab257758) was used. Wild-type and TPM4 knockout samples were subjected to SDS-PAGE. ab181156 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TPM4 antibody [EPR13317] (<a href='/en-us/products/primary-antibodies/tpm4-antibody-epr13317-ab181156'>ab181156</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

TPM4 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human TPM4 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-tpm4-knockout-hek-293t-cell-line-ab266606'>ab266606</a>)

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 28 kDa

Observed band size: 29 kDa

false

Western blot - Human TPM4 knockout HEK-293T cell lysate (AB257758)
  • WB

Lab

Western blot - Human TPM4 knockout HEK-293T cell lysate (AB257758)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : TPM4 knockout HEK293T cell lysate (20 ug)
Lane 3 : HeLa cell lysate (20 ug)

ab181085 was shown to specifically react with TPM4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266606 (knockout cell lysate ab257758) was used. Wild-type and TPM4 knockout samples were subjected to SDS-PAGE. ab181085 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TPM4 antibody [EPR13316] (<a href='/en-us/products/primary-antibodies/tpm4-antibody-epr13316-ab181085'>ab181085</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

TPM4 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human TPM4 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-tpm4-knockout-hek-293t-cell-line-ab266606'>ab266606</a>)

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 28 kDa

Observed band size: 29 kDa

false

Sanger Sequencing - Human TPM4 knockout HEK-293T cell lysate (AB257758)
  • Sanger seq

Unknown

Sanger Sequencing - Human TPM4 knockout HEK-293T cell lysate (AB257758)

Allele-2 : Insertion of the selection cassette in exon 1

Sanger Sequencing - Human TPM4 knockout HEK-293T cell lysate (AB257758)
  • Sanger seq

Unknown

Sanger Sequencing - Human TPM4 knockout HEK-293T cell lysate (AB257758)

Allele-1 : 1 bp insertion in exon 1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and Insertion of the selection cassette in exon 1.

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
TPM4
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TPM4 also known as tropomyosin 4 or TM4 is a protein that helps regulate muscle contraction and stabilize actin filaments. It has a molecular mass of approximately 28 kDa. TPM4 expression occurs in various tissues including both skeletal and smooth muscle tissues as well as in non-muscle cells. This protein is part of a larger family of tropomyosins which bind to actin filaments and play an important role in modulating actin's interaction with other proteins.
Biological function summary

TPM4 contributes to the regulation of the actin cytoskeleton dynamics influencing cell movement and shape. TPM4 like other tropomyosins associates with actin filaments and forms a part of a complex with other actin-binding proteins such as tropomodulins. This complex is critical in both maintaining filament stability and facilitating the sliding of actin filaments during muscle contraction and other cellular activities.

Pathways

TPM4 participates actively in the cytoskeletal signaling and muscle contraction pathways. It interacts with important components in these pathways such as myosin allowing coordinated muscle contractions. TPM4 also plays a role in the regulation of the RhoA signaling pathway which is connected to cytoskeletal rearrangement and cell motility. These interactions highlight TPM4's integral position in biological processes relying on actin filament dynamics.

Misregulation of TPM4 can influence the development of conditions such as cardiomyopathies and cancer. Alterations in its expression or function may link to familial hypertrophic cardiomyopathy where it interacts with cardiac-specific proteins like cardiac myosin heavy chain (MYH7). Furthermore changes in TPM4 expression levels may associate with cancer metastasis in part due to its role in cell motility and invasion pointing to potential connections with proteins like cofilin during cancer progression.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

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