Human TRABD (TraB domain containing) knockout HEK-293T cell lysate

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TraB domain containing protein functions mechanically as a facilitator for DNA transfer during bacterial conjugation. This protein aids in the transport of DNA across the cell membrane playing a critical role in gene exchange processes. Often referred to by various names such as conjugation protein TraB or transfer binding domain protein it has an estimated mass around 50 kDa. TraB is typically expressed in bacteria where it ensures the proper transfer of genetic material between cells harnessing energy from ATP to drive this process efficiently.

Biological function summary

TraB domain containing proteins enable horizontal gene transfer which significantly impacts genetic diversity and adaptation in bacterial populations. It constitutes a part of the larger conjugative transfer machinery complex that involves multiple proteins. This complex facilitates the binding and unwinding of DNA which is essential for conjugation. TraB works in association with membrane-associated proteins to ensure successful DNA passage into the recipient cell reflecting its importance in cellular adaptability and survival under various environmental conditions.

Pathways

These proteins serve a vital function within the bacterial conjugation pathway — a fundamental mechanism contributing to antibiotic resistance spread. TraB operates in coordination with proteins such as TraC or TraG which form part of the transfer complex. These proteins orchestrate the conjugation process ensuring the precise transfer of DNA molecules working as a liaison among the numerous proteins involved in this method. The integration of TraB within these pathways demonstrates its role in promoting gene transfer which bacteria use to acquire new traits rapidly including antibiotic resistance.

TraB-related processes have considerable implications on the progression of antibiotic resistance in bacterial infections. The spread of antibiotic-resistant genes through TraB-mediated conjugation poses significant challenges in treating diseases like MRSA (Methicillin-resistant Staphylococcus aureus). TraB connects with other proteins like TraA or TraD facilitating resistance gene dissemination within bacterial communities. Understanding TraB's function and its interactions with these proteins is essential in developing strategies to combat the growing public health threat posed by antibiotic-resistant bacterial strains.