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AB257759

Human TRAF2 knockout HEK-293T cell lysate

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TRAF2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 7 bp deletion in exon 2.

View Alternative Names

E3 ubiquitin-protein ligase TRAF2, MGC:45012, OTTHUMP00000022625, OTTHUMP00000064745, TNF receptor-associated factor 2, TNF receptor-associated protein, TR AP, TRAF2_HUMAN, TRAP 3, Tumor necrosis factor type 2 receptor-associated protein 3

3 Images
Western blot - Human TRAF2 knockout HEK-293T cell lysate (AB257759)
  • WB

Lab

Western blot - Human TRAF2 knockout HEK-293T cell lysate (AB257759)

Lane 1 : Wild-type HEK-293T cell lysate (20µg)

Lane 2 : TRAF2 knockout HEK-293T cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab126758 observed at 55 kDa. Red - loading control ab8245 observed at 37 kDa.

ab126758 Anti-TRAF2 antibody [EPR6048] was shown to specifically react with TRAF2 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266060 (knockout cell lysate ab257759) was used. Wild-type and TRAF2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab126758 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TRAF2 antibody [EPR6048] (<a href='/en-us/products/primary-antibodies/traf2-antibody-epr6048-ab126758'>ab126758</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

TRAF2 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human TRAF2 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-traf2-knockout-hek-293t-cell-line-ab266060'>ab266060</a>)

Predicted band size: 55 kDa

Observed band size: 55 kDa

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Western blot - Human TRAF2 knockout HEK-293T cell lysate (AB257759)
  • WB

Lab

Western blot - Human TRAF2 knockout HEK-293T cell lysate (AB257759)

Lane 1 : Wild-type HEK-293T cell lysate (20µg)

Lane 2 : TRAF2 knockout HEK-293T cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab167163 observed at 55 kDa. Red - loading control ab8245 observed at 37 kDa.

ab167163 Anti-TRAF2 antibody [EPR7064] was shown to specifically react with TRAF2 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266060 (knockout cell lysate ab257759) was used. Wild-type and TRAF2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab167163 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TRAF2 antibody [EPR7064] (<a href='/en-us/products/primary-antibodies/traf2-antibody-epr7064-ab167163'>ab167163</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

TRAF2 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human TRAF2 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-traf2-knockout-hek-293t-cell-line-ab266060'>ab266060</a>)

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

Sanger Sequencing - Human TRAF2 knockout HEK-293T cell lysate (AB257759)
  • Sanger seq

Unknown

Sanger Sequencing - Human TRAF2 knockout HEK-293T cell lysate (AB257759)

Homozygous : 7 bp deletion in exon 2

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 7 bp deletion in exon 2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
TRAF2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAF2 known also as TNF Receptor-Associated Factor 2 is an adapter protein weighing approximately 56 kDa. It is expressed in many tissues such as lymph nodes spleen and thymus. This protein acts as a signal transducer playing an integral role in the activation of NF-kB and MAPK signaling pathways. TRAF2 interacts with TNF receptors and other proteins using its RING finger and zinc finger domains for ubiquitination processes.
Biological function summary

TRAF2 modulates immune responses by regulating cell survival proliferation and apoptosis. It often forms complexes with other TRAF family proteins to mediate signal transmission. These complexes control the balance between pro-apoptotic and anti-apoptotic signals impacting the regulation of immune and inflammatory responses. Its interactions are necessary for the proper function of NF-kB ensuring balanced immune activities.

Pathways

TRAF2 is a critical component within both NF-kB and JNK signaling pathways. In the NF-kB pathway TRAF2 intersects with proteins like IKK and NEMO facilitating the transcription of genes involved in immune and inflammatory responses. In JNK signaling TRAF2 influences cellular responses through regulation of apoptotic signals. By linking these pathways TRAF2 ensures a coordinated cellular response to extracellular stimuli.

TRAF2’s dysregulation is linked with autoimmune diseases and certain types of cancer. Overactivity can contribute to chronic inflammation highlighting its role in autoimmune disorders like rheumatoid arthritis. In cancer TRAF2’s involvement with TNF receptors can lead to uncontrolled cell proliferation. Proteins like cIAP1 and cIAP2 are often related to TRAF2 in these contexts influencing the progression and modulation of disease states.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Primary Antibodies

AB126758

Anti-TRAF2 antibody [EPR6048]

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Flow Cytometry (Intracellular) - Anti-TRAF2 antibody [EPR6048] (AB126758)

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Proteins & Peptides

AB204091

Recombinant Human TRAF2 protein

SDS-PAGE - Recombinant Human TRAF2 protein (AB204091)

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Assay Kits

AB226893

Human TRAF2 ELISA kit

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Sandwich ELISA - Human TRAF2 ELISA kit (AB226893)

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Primary Antibodies

AB108601

Anti-FADD antibody [EPR4415]

RabMAb|Recombinant|KO Validated

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Product promise

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