Human TRANK1 knockout A549 cell lysate
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TRANK1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon11.
View Alternative Names
KIAA0342, LBA1, Lupus brain antigen 1 homolog, TPR and ankyrin repeat-containing protein 1, TRNK1_HUMAN
- Sanger seq
Unknown
Sanger Sequencing - Human TRANK1 knockout A549 cell lysate (AB259203)
Homozygous : 1 bp insertion in exon11
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Several cellular activities rely on TRANK1 due to its ability to participate in multiprotein complexes. It regulates transcription and cell cycle processes impacting how cells grow and respond to environmental signals. By modulating the activity of other proteins TRANK1 enables cells to maintain homeostasis and adapt to changes. These actions suggest a role in normal brain functions where regulatory precision is needed.
Pathways
The TRANK1 protein interacts within the Wnt signaling and stress response pathways. These pathways involve proteins that regulate gene expression in response to extracellular signals affecting cell fate and proliferation. In the Wnt signaling pathway TRANK1 can influence components like β-catenin impacting processes like cell differentiation. It ensures proper signaling flow affecting cellular responses and maintaining healthy cellular states.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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