Human TREM2 knockout THP-1 cell lysate
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TREM2 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9; X = 1 bp deletion, 2 bp deletion; Frameshift: 97%.
View Alternative Names
TREM2_HUMAN, TREM2a, TREM2b, TREM2c, Trggering receptor expressed on myeloid cells 2, Trggering receptor expressed on myeloid cells 2a, Triggering receptor expressed on monocytes 2, Triggering receptor expressed on myeloid cells 2
- WB
Lab
Western blot - Human TREM2 knockout THP-1 cell lysate (AB269652)
Lane 1 : Wild-type THP-1 cell lysate 20 μg
Lane 2 : TREM2 knockout THP-1 cell lysate 20 μg
Lane 3 : HL-60 cell lysate 20 μg
Lanes 1 - 3 : Merged signal (red and green). Green - ab209814 observed at 30 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab209814 was shown to react with TREM2 in wild-type THP-1 cells in Western blot with loss of signal observed in TREM2 knockout cell line ab269489 (TREM2 knockout cell lysate ab269652). Wild-type THP-1 and TREM2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab209814 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes:
Western blot - Anti-TREM2 antibody [EPR20243] (<a href='/en-us/products/primary-antibodies/trem2-antibody-epr20243-ab209814'>ab209814</a>) at 1/1000 dilution
Lane 1:
Wild-type THP-1 cell lysate at 20 µg
Lane 2:
Western blot - Human TREM2 knockout THP-1 cell lysate (ab269652) at 20 µg
Lane 3:
HL-60 cell lysate at 20 µg
Predicted band size: 25 kDa
Observed band size: 30 kDa
false
- NGS
Supplier Data
Next Generation Sequencing - Human TREM2 knockout THP-1 cell lysate (AB269652)
Homozygous 2bp deletion in exon 2.
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Suspension
Gender
Male
Complementary Products
Primary Antibodies
AB209814
Anti-TREM2 antibody [EPR20243]
20ul selling size|RabMAb|Recombinant|KO Validated
![Immunoprecipitation - Anti-TREM2 antibody [EPR20243] (AB209814)](https://content.abcam.com/products/images/trem2-antibody-epr20243-ab209814--immunoprecipitation-img15942.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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