Human TRIM14 knockout A549 cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human TRIM14 knockout A549 cell lysate (AB259205)
Allele-3 : 1 bp deletion in exon4
- Sanger seq
Unknown
Sanger Sequencing - Human TRIM14 knockout A549 cell lysate (AB259205)
Allele-1 : 23 bp deletion in exon4
- Sanger seq
Unknown
Sanger Sequencing - Human TRIM14 knockout A549 cell lysate (AB259205)
Allele-2 : 4 bp deletion in exon4
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TRIM14 plays a role in innate immune response by modulating signaling pathways. It does not have E3 ubiquitin ligase activity like other TRIM family members but interacts with proteins in antiviral defenses. TRIM14 associates with the mitochondria contributing to the formation of complexes with proteins like MAVS which are involved in the antiviral response to RNA viruses.
Pathways
TRIM14 is integral to the regulation of innate immune signaling pathways. It participates in the RIG-I-like receptor signaling pathway essential for detecting viral RNA. TRIM14 does this through interaction with MAVS leading to the activation of downstream signaling proteins including NF-kB and IRF3. These proteins result in the production of type I interferons and pro-inflammatory cytokines.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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