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AB257767

Human TRIM21 (SS-A) knockout A549 cell lysate

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TRIM21 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon4 and 1 bp insertion in exon4.

View Alternative Names

52 kDa Ro protein, 52 kDa ribonucleoprotein autoantigen Ro/SS-A, TRIM21, RING finger protein 81, Sjoegren syndrome type A antigen, Ro(SS-A), RNF81, SS-A, SSA1, Tripartite motif-containing protein 21, RO52, E3 ubiquitin-protein ligase TRIM21

4 Images
Western blot - Human TRIM21 (SS-A) knockout A549 cell lysate (AB257767)
  • WB

Supplier Data

Western blot - Human TRIM21 (SS-A) knockout A549 cell lysate (AB257767)

ab207728 was shown to react with TRIM21 in wild-type A549 cells in Western blot with loss of signal observed in TRIM21 knockout cell line ab267025. Wild-type A549 and TRIM21 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab207728 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-TRIM21/SS-A antibody [EPR20290] (<a href='/en-us/products/primary-antibodies/trim21-ss-a-antibody-epr20290-ab207728'>ab207728</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 lysate at 30 µg

Lane 2:

TRIM21 knock-out A549 lysate at 30 µg

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Western blot - Human TRIM21 (SS-A) knockout A549 cell lysate (AB257767)
  • WB

Unknown

Western blot - Human TRIM21 (SS-A) knockout A549 cell lysate (AB257767)

Lane 1 : Wild-type A549 cell lysate (20 ug)
Lane 2 : TRIM21 knockout A549 cell lysate (20 ug)
Lane 3 : HeLa cell lysate (20 ug)
Lane 4 : MOLT-4 cell lysate (20 ug)

ab207728 was shown to specifically react with TRIM21/SS-A in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267025 (knockout cell lysate ab257767) was used. Wild-type and TRIM21/SS-A knockout samples were subjected to SDS-PAGE. ab207728 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

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Sanger Sequencing - Human TRIM21 (SS-A) knockout A549 cell lysate (AB257767)
  • Sanger seq

Unknown

Sanger Sequencing - Human TRIM21 (SS-A) knockout A549 cell lysate (AB257767)

Allele-2 : 1 bp insertion in exon4

Sanger Sequencing - Human TRIM21 (SS-A) knockout A549 cell lysate (AB257767)
  • Sanger seq

Unknown

Sanger Sequencing - Human TRIM21 (SS-A) knockout A549 cell lysate (AB257767)

Allele-1 : 13 bp deletion in exon4

Key facts

Cell type

A549

Species or organism

Human

Tissue

Lung

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon4 and 1 bp insertion in exon4.

Disease

Carcinoma

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Complementary Products

Primary Antibodies

AB207728

Anti-TRIM21/SS-A antibody [EPR20290]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM21/SS-A antibody [EPR20290] (AB207728)

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Primary Antibodies

AB216347

Anti-SNAIL antibody [EPR21043]

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
TRIM21
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIM21 also known as SS-A or Ro52 is a 52 kDa protein that plays a role in the immune system. It functions as an E3 ubiquitin ligase involved in the ubiquitination process. This protein is expressed in various tissues including immune cells like lymphocytes and macrophages. TRIM21 recognizes autoantibodies linked to TRIM21 activity particularly those targeting the SS-A complex. Researchers often use antibodies like anti-TRIM21 or anti-SS-A to study its interactions and effects in cellular processes.
Biological function summary

TRIM21 contributes significantly to the regulation of immune responses and participates in innate and adaptive immunity. It forms part of a complex with other proteins to facilitate the degradation of viral particles through the ubiquitin-proteasome system a process known as antibody-dependent intracellular neutralization. As an important player in the immune defense TRIM21 oversees the timely removal of pathogens and prevents potential overactivation of immune responses that might harm the host.

Pathways

TRIM21 operates within the interferon signaling and NF-κB pathways two important areas of immune response modulation. TRIM21 interacts with molecules like transcription factors that influence the expression of interferon-responsive genes which are critical for pathogen defense. Its role in these pathways highlights its interactions with various immune-regulatory proteins helping to maintain immune system balance and effectiveness during infections.

TRIM21 has strong associations with autoimmune conditions such as systemic lupus erythematosus and Sjögren's syndrome. Autoantibodies against TRIM21 are often used as biomarkers for these diseases and their presence can indicate increased autoimmunity. Relationship with other autoantigens like SS-A/Ro60 further connects TRIM21 with disease mechanisms suggesting its importance in the pathology of these autoimmune disorders. Understanding the role of TRIM21 in these diseases may improve diagnostic and therapeutic strategies.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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