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AB258248

Human TRIM34 (RNF21/IFP1) knockout A549 cell lysate

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TRIM34 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon8 and 20 bp deletion in exon8.

View Alternative Names

IFP1, Interferon Responsive Finger Protein 1, RNF21, Ring Finger Protein 21, Ring finger protein 21 interferon responsive, TRIM 34, Tripartite motif containing protein 34

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Sanger Sequencing - Human TRIM34 (RNF21/IFP1) knockout A549 cell lysate (AB258248)
  • Sanger seq

Unknown

Sanger Sequencing - Human TRIM34 (RNF21/IFP1) knockout A549 cell lysate (AB258248)

Allele-2 : 1 bp insertion in exon8

Sanger Sequencing - Human TRIM34 (RNF21/IFP1) knockout A549 cell lysate (AB258248)
  • Sanger seq

Unknown

Sanger Sequencing - Human TRIM34 (RNF21/IFP1) knockout A549 cell lysate (AB258248)

Allele-1 : 20 bp deletion in exon8

Key facts

Cell type

A549

Species or organism

Human

Tissue

Lung

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon8 and 20 bp deletion in exon8.

Disease

Carcinoma

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
TRIM34
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF21 also known as IFP1 is a human protein with a molecular weight of approximately 43 kDa. It belongs to the RING finger protein family functioning as an E3 ubiquitin ligase. Members of this family typically regulate protein degradation through the ubiquitin-proteasome system. RNF21 is widely expressed in various tissues including the brain liver and heart. This expression suggests important roles across different physiological contexts.
Biological function summary

The E3 ubiquitin ligase activity of RNF21 contributes to the regulation of protein homeostasis. RNF21 potentially participates in forming larger protein complexes which could be involved in cellular processes like cell cycle regulation. By tagging specific proteins for degradation RNF21 maintains cellular protein equilibrium. This process impacts various cellular functions including signal transduction and cellular stress responses.

Pathways

The function of RNF21 integrates within the ubiquitin-proteasome pathway and connects to protein turnover. This pathway involves numerous proteins such as ubiquitin-conjugating enzymes which work alongside RNF21. Additionally RNF21 may intersect with signaling pathways related to protein stability where it collaborates with proteins like SCF complexes which also function in protein degradation.

RNF21 has connections to neurodegenerative diseases such as Alzheimer's disease where protein aggregation becomes problematic. Aberrant protein degradation can exacerbate these disease conditions. RNF21's interactions with proteins like tau which is involved in Alzheimer's pathology illustrate its potential influence. Moreover its role in inflammatory responses might link RNF21 to disorders like rheumatoid arthritis where improper protein regulation plays a role.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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Product promise

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For full details, please see our Terms & Conditions

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