JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB258249

Human TRIM56 knockout A549 cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

TRIM56 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and 2 bp deletion in exon3.

View Alternative Names

A130009K11Rik, DKFZp667O116, E3 ubiquitin-protein ligase TRIM56, FLJ35608, Gm452, MGC37358, OTTMUSP00000027392, RING finger protein 109, RNF109, TRI56_HUMAN, Tripartite motif-containing protein 56

4 Images
Western blot - Human TRIM56 knockout A549 cell lysate (AB258249)
  • WB

Lab

Western blot - Human TRIM56 knockout A549 cell lysate (AB258249)

Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate (20 ug)
Lane 2 : TRIM56 knockout A549 (Human lung carcinoma cell line) whole cell lysate (20 ug)
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate (20 ug)
Lane 4 : A-375 (Human malignant melanoma cell line) whole cell lysate (20 ug)

ab154821 was shown to specifically react with TRIM56 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267062 (knockout cell lysate ab258249) was used. Wild-type and TRIM56 knockout samples were subjected to SDS-PAGE. ab154821 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TRIM56 antibody [EPR10582] (<a href='/en-us/products/primary-antibodies/trim56-antibody-epr10582-ab154821'>ab154821</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

TRIM56 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human TRIM56 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-trim56-knockout-a549-cell-line-ab267062'>ab267062</a>)

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 4:

A-375 (Human malignant melanoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 81 kDa

Observed band size: 88 kDa

false

Western blot - Human TRIM56 knockout A549 cell lysate (AB258249)
  • WB

Lab

Western blot - Human TRIM56 knockout A549 cell lysate (AB258249)

Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate (20 ug)
Lane 2 : TRIM56 knockout A549 (Human lung carcinoma cell line) whole cell lysate (20 ug)
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate (20 ug)
Lane 4 : A-375 (Human malignant melanoma cell line) whole cell lysate (20 ug)

ab154862 was shown to specifically react with TRIM56 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267062 (knockout cell lysate ab258249) was used. Wild-type and TRIM56 knockout samples were subjected to SDS-PAGE. ab154862 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TRIM56 antibody [EPR10583] (<a href='/en-us/products/primary-antibodies/trim56-antibody-epr10583-ab154862'>ab154862</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

TRIM56 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human TRIM56 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-trim56-knockout-a549-cell-line-ab267062'>ab267062</a>)

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 4:

A-375 (Human malignant melanoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 81 kDa

Observed band size: 88 kDa

false

Sanger Sequencing - Human TRIM56 knockout A549 cell lysate (AB258249)
  • Sanger seq

Unknown

Sanger Sequencing - Human TRIM56 knockout A549 cell lysate (AB258249)

Allele-1 : 2 bp deletion in exon3

Sanger Sequencing - Human TRIM56 knockout A549 cell lysate (AB258249)
  • Sanger seq

Unknown

Sanger Sequencing - Human TRIM56 knockout A549 cell lysate (AB258249)

Allele-2 : 1 bp deletion in exon3

Key facts

Cell type

A549

Species or organism

Human

Tissue

Lung

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and 2 bp deletion in exon3.

Disease

Carcinoma

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258249-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human TRIM56 knockout A549 cell lysate", "number":"AB258249-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type A549 cell lysate", "number":"AB258249-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
TRIM56
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIM56 also known as Tripartite Motif Containing 56 or RNF109 is a protein involved in various cellular mechanisms. It weighs around 79 kDa and is expressed in many tissues including the spleen lymph nodes and lungs. TRIM56 contains a RING finger domain B-box motifs and a coiled-coil region which are typical features of the TRIM protein family. These domains suggest roles in ubiquitination and protein-protein interactions which are essential for its functions in signaling and regulatory processes.
Biological function summary

TRIM56 participates in immune regulation and antiviral defense. It acts as an interferon-stimulated gene product and does not appear to be part of a larger protein complex. Instead it induces antiviral states by mediating the innate immune response to viral infections. TRIM56 enhances the production of type I interferons by modifying the activation pathways. This function places TRIM56 as an immune defense agent which can restrict the replication of various viruses such as flaviviruses and coronaviruses therefore harboring significant implications in pathogen defense responses.

Pathways

TRIM56 integrates into the antiviral and innate immunity pathways. It prominently situates within the interferon signaling pathway enhancing the body's defense through the upregulation of interferon production. TRIM56 interacts with several proteins in these pathways including STING and MAVS which are known to be important mediators in the recognition of viral presence. Through these interactions TRIM56 facilitates the activation of downstream signaling cascades leading to the expression of interferon-stimulated genes.

TRIM56 exhibits significant connections to viral infections and autoimmune diseases. Its role in antiviral responses implicates it in diseases like hepatitis C as it can suppress viral replication. Although TRIM56 aids in defending against infections its dysregulation may also link to autoimmune conditions where its interaction with proteins such as IFNAR1 can result in excessive immune responses leading to tissue damage and inflammation. Such dual roles highlight TRIM56 as an important regulator in both protective and pathological immune responses.
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB269548

Human IL1R2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human IL1R2 ELISA Kit (AB269548)

  • 0
  • 0
Primary Antibodies

AB35000

Anti-Dexamethasone antibody

  • 0
  • 0
Primary Antibodies

AB154862

Anti-TRIM56 antibody [EPR10583]

RabMAb|Recombinant|KO Validated

Western blot - Anti-TRIM56 antibody [EPR10583] (AB154862)

  • 0
  • 0
Assay Kits

AB113851

DCFDA / H2DCFDA - Cellular ROS Assay Kit

Cellular Activity - DCFDA / H2DCFDA - Cellular ROS Assay Kit (AB113851)

  • 5
  • 11

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com