Human TRIP13 (PCH2) knockout HEK-293T cell lysate
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TRIP13 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
View Alternative Names
16E1-BP, HPV16 E1 protein-binding protein, Homo sapiens HPV16 E1 protein binding protein mRNA complete cds, Human papillomavirus type 16 E1 protein-binding protein, Pachytene checkpoint protein 2 homolog, TR-interacting protein 13, TRIP-13, TRP13_HUMAN, Thyroid hormone receptor interactor 13, Thyroid receptor-interacting protein 13
- WB
Lab
Western blot - Human TRIP13 (PCH2) knockout HEK-293T cell lysate (AB258736)
Anti-TRIP13 antibody (ab128171) staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab128171 was shown to bind specifically to TRIP13. A band was observed at 48 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in TRIP13 knockout cell line. To generate this image, wild-type and TRIP13 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes:
Western blot - Anti-TRIP13/PCH2 antibody (<a href='/en-us/products/primary-antibodies/trip13-pch2-antibody-ab128171'>ab128171</a>) at 1/2000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human TRIP13 (PCH2) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-trip13-pch2-knockout-hek-293t-cell-line-ab266495'>ab266495</a>)
Lane 2:
TRIP13 knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human TRIP13 (PCH2) knockout HEK-293T cell lysate (ab258736)
Lane 3:
HCT 116 cell lysate at 20 µg
Lane 4:
SW480 cell lysate at 20 µg
Predicted band size: 49 kDa
Observed band size: 48 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human TRIP13 (PCH2) knockout HEK-293T cell lysate (AB258736)
Allele-2 : Insertion of the selection cassette in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human TRIP13 (PCH2) knockout HEK-293T cell lysate (AB258736)
Allele-1 : 17 bp deletion in exon 1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TRIP13 contributes to efficient cell cycle progression by participating in the spindle assembly checkpoint (SAC). It forms part of the mitotic checkpoint complex ensuring that chromosomes are correctly attached to the spindle microtubules before progression through mitosis. TRIP13 works in conjunction with other proteins like MAD2 and BUB1 to maintain genomic stability.
Pathways
TRIP13 is involved in the cell cycle control and DNA repair pathways. It influences the mitotic spindle checkpoint interacting with proteins such as MAD2 which ensures that cells do not proceed to anaphase until all chromosomes reach proper alignment. TRIP13 also plays a role in homologous recombination repair by regulating the processing of recombination intermediates.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com