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TRMT1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon 6.

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Images

Western blot - Human TRMT1 (TRM1) knockout HEK-293T cell lysate (AB258252), expandable thumbnail
  • Western blot - Human TRMT1 (TRM1) knockout HEK-293T cell lysate (AB258252), expandable thumbnail
  • Sanger Sequencing - Human TRMT1 (TRM1) knockout HEK-293T cell lysate (AB258252), expandable thumbnail
  • Sanger Sequencing - Human TRMT1 (TRM1) knockout HEK-293T cell lysate (AB258252), expandable thumbnail

Key facts

Cell type
HEK-293T
Species or organism
Human
Tissue
Kidney
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon 6.

Alternative names

2)G26)dimethyltransferase, 2-dimethylguanosine-26 methyltransferase, FLJ20244, N(2) N(2) dimethylguanosine tRNA methyltransferase, N(2)-N(2)) methyltransferase, TRM1 tRNA methyltransferase 1 homolog, TRM1_HUMAN, TRMT 1, tRNA (guanine(26)-N(2))-dimethyltransferase, tRNA 2, tRNA 2 2 dimethylguanosine 26 methyltransferase, tRNA(guanine 26 N(2) N(2)) methyltransferase, tRNA(guanine-26, tRNA(m(2, tRNA(m(2 2)G26)dimethyltransferase

What's included?

1 Kit
Components
Human TRMT1 knockout HEK293T cell lysate
1 x 100 µg
Human wild-type HEK293T cell lysate
1 x 100 µg

Recommended products

TRMT1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon 6.

Key facts

Cell type
HEK-293T
Mutation description
Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon 6.
Concentration
Loading...

Properties

Gene name
TRMT1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Notes


Knockout cell lysate achieved by CRISPR/Cas9.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TRM1 also known as transfer RNA methyltransferase 1 is an enzyme responsible for methylating the N2 position of guanine in tRNA molecules. It has a molecular mass of approximately 49 kDa. TRM1 mostly resides in the mitochondria but can also be found in the cytoplasm. Its presence ensures proper methylation of tRNA influencing efficient and accurate protein translation.

Biological function summary

The methylation activity of TRM1 plays a role in maintaining the stability and function of tRNA molecules. As part of the cellular machinery TRM1 does not function alone; it often works together with other tRNA-modifying enzymes to ensure the fidelity of protein synthesis. This modification protects tRNA's structure facilitates proper folding and enhances interaction with ribosomal components.

Pathways

TRM1 functions significantly impact protein synthesis pathways. The enzyme is instrumental in the translation process by ensuring that tRNA molecules are correctly modified for protein assembly. TRM1 operates alongside other proteins such as TRM10 and TRMT5 within these modification pathways influencing the efficiency and accuracy of translation. It supports processes like tRNA maturation and ribosomal activity.

Associated diseases and disorders

Alterations in TRM1 activity can relate to mitochondrial pathologies. Mutations or deficiencies in TRM1 can disrupt mitochondrial function leading to disorders such as mitochondrial myopathy. Research also connects TRM1 aberrations to neurodegenerative diseases where it may interact with proteins like PINK1 highlighting its role in neuroprotective responses. The efficiency of TRM1 in performing its tasks is vital as any dysfunction can result in profound cellular consequences affecting human health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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