Human TROAP knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human TROAP knockout HEK-293T cell lysate (AB259211)
Allele-1 : Insertion of the selection cassette in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human TROAP knockout HEK-293T cell lysate (AB259211)
Allele-3 : 2 bp insertion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human TROAP knockout HEK-293T cell lysate (AB259211)
Allele-2 : Insertion of the selection cassette in exon2
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Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TROAP serves roles in establishing cell-cell contact and adhesion during implantation. It is part of a complex with trophinin bystin and tastin working together to mediate cell adhesion at the embryo-maternal interface. TROAP supports the binding between cells enabling proper communication and anchoring essential during early embryonic development and trophoblastic invasion.
Pathways
The involvement of TROAP in cell adhesion links it to key reproductive and developmental pathways. It is part of pathways involving cell-to-cell junctions wherein its interaction with trophinin contributes to the adhesion mechanism. Related proteins in these pathways include bystin and tastin which further strengthen the adhesion complex promoting effective embryonic implantation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com