Human TRPM7 knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human TRPM7 knockout HeLa cell lysate (AB259212)
Allele-3 : 1 bp deletion in exon14
- Sanger seq
Unknown
Sanger Sequencing - Human TRPM7 knockout HeLa cell lysate (AB259212)
Allele-1 : 8 bp deletion in exon14
- Sanger seq
Unknown
Sanger Sequencing - Human TRPM7 knockout HeLa cell lysate (AB259212)
Allele-2 : 5 bp deletion in exon14
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TRPM7 regulates cellular magnesium homeostasis and calcium influx in many cell types. It is integral in maintaining the cell's ion balance and influences various cellular functions like growth proliferation and apoptosis. TRPM7 is often part of larger molecular complexes that enable it to interact with other proteins and lipids modulating signal transduction pathways. It participates in mechanosensory functions as well impacting cellular responses to mechanical stimuli.
Pathways
TRPM7 is significant in the MAPK and PI3K/Akt pathways which are pivotal for cell survival and proliferation. Through these pathways TRPM7 interacts with other proteins such as TRPM6 and TRPV4. It modulates intracellular signaling cascades by regulating ion concentrations that affect protein interactions and enzyme activities thereby controlling critical cellular responses like inflammation and differentiation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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