TSPO KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 2 and 5 bp deletion in exon 2.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 2 and 5 bp deletion in exon 2.
Alternative names
BPBS, BZRP, Benzodiazapine receptor (peripheral), Benzodiazepine peripheral binding site, IBP, Isoquinoline carboxamide-binding protein, MBR, Mitochondrial benzodiazepine receptor, PKBS, PTBR, PTBZR02, Peripheral benzodiazepine receptor, Peripheral benzodiazepine receptor-related protein, Peripheral-type benzodiazepine receptor, Ptbzr, RATPTBZR02, TSPOA_HUMAN, Translocator protein, Tspo1, mDRC, pk18, translocator protein (18kDa)
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TSPO KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 2 and 5 bp deletion in exon 2.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 2 and 5 bp deletion in exon 2.
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- TSPO
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing, Western blot
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PBR also known as the Peripheral Benzodiazepine Receptor is a protein predominantly found in the outer mitochondrial membrane. It has alternate names like Translocator Protein (TSPO) and the mass of this protein is approximately 18 kDa. PBR is expressed in various tissues but shows high levels in steroidogenic tissues like adrenal glands as well as in the brain heart liver and kidneys. The abundant presence in these tissues highlights its importance in a variety of physiological functions.
- Biological function summary
PBR interacts with cholesterol for the synthesis of steroid hormones making it important for steroidogenesis. PBR is a part of the Mitochondrial Permeability Transition Pore complex (MPTP) involved in regulating the transport of molecules across the mitochondrial membrane. Through its association with the MPTP PBR plays a significant role in mitochondrial functions such as apoptosis and energy metabolism. The interaction with other molecules also includes the binding with benzodiazepines impacting processes like immune response and cell proliferation.
- Pathways
PBR plays a role in the steroid biosynthesis and apoptosis pathways. It interfaces with the StAR (Steroidogenic Acute Regulatory) protein to facilitate cholesterol transport into mitochondria the initial step in steroid hormone production. PBR is also involved in pathways that regulate apoptosis and mitochondrial function linking it to different cellular processes through interactions with proteins like VDAC (Voltage-Dependent Anion Channel).
- Associated diseases and disorders
PBR has relevance to conditions such as neurodegenerative diseases and cancer. PBR expression changes in disorders like Alzheimer's disease where it might reflect mitochondrial dysfunctions. It has also been associated with certain cancers where aberrant PBR activity might contribute to altered cell proliferation and apoptosis. Proteins like caspases are involved in the apoptotic pathways connected with PBR highlighting its involvement in disease processes.
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3 product images
Western blot - Human TSPO (PBR) knockout HeLa cell lysate (ab257066)
Lane 1: Wild-type HeLa cell lysate (20μg)
Lane 2: TSPO knockout HeLa cell lysate (20μg)
Lanes 1- 2: Merged signal (red and green). Green - Anti-PBR antibody [EPR5384] ab109497 observed at 15 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-PBR antibody [EPR5384] ab109497 Anti-PBR antibody [EPR5384] was shown to specifically react with PBR in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human TSPO (PBR) knockout HeLa cell line ab264942 (knockout cell lysate ab257066) was used. Wild-type and PBR knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-PBR antibody [EPR5384] ab109497 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-PBR antibody [EPR5384] (Anti-PBR antibody [EPR5384] ab109497) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: TSPO (PBR) knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human TSPO (PBR) knockout HeLa cell line (Human TSPO (PBR) knockout HeLa cell line ab264942)
Performed under reducing conditions.
Predicted band size: 112 kDa, 13 kDa, 134 kDa, 152 kDa, 17 kDa, 19 kDa, 25 kDa, 26 kDa, 28 kDa, 29 kDa, 37 kDa, 38 kDa, 39 kDa, 42 kDa, 43 kDa, 49 kDa, 50 kDa, 52 kDa, 56 kDa, 58 kDa, 68 kDa, 69 kDa, 71 kDa, 72 kDa, 74 kDa, 80 kDa, 81 kDa, 83 kDa, 87 kDa
Observed band size: 112 kDa, 15 kDa, 36 kDa, 38 kDa, 42 kDa, 45 kDa, 55 kDa, 60 kDa, 74 kDa, 80 kDa, 87 kDa
Sanger Sequencing - Human TSPO (PBR) knockout HeLa cell lysate (ab257066)
Allele-2: 2 bp deletion in exon 2
Sanger Sequencing - Human TSPO (PBR) knockout HeLa cell lysate (ab257066)
Allele-1: 5 bp deletion in exon 2
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