Human TSSC1 knockout HeLa cell lysate
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EIPR1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4 and Insertion of the selection cassette in exon4.
View Alternative Names
Tumor suppressing STF cDNA 1 protein, Tumor suppressing subchromosomal transferable fragment, Tumor suppressing subchromosomal transferable fragment candidate gene 1 protein, Tumor suppressing subtransferable candidate 1
- Sanger seq
Unknown
Sanger Sequencing - Human TSSC1 knockout HeLa cell lysate (AB263399)
Allele-2 : Insertion of the selection cassette in exon4
- Sanger seq
Unknown
Sanger Sequencing - Human TSSC1 knockout HeLa cell lysate (AB263399)
Allele-1 : 1 bp insertion in exon4
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TSSC1 contributes to cellular homeostasis and development. It is part of the Commander complex which is important for endosomal sorting and trafficking. By aiding the assembly and stabilization of this complex TSSC1 supports cellular processes that rely on proper endosomal transport. This function maintains cellular activities that involve the movement and sorting of various biomolecules impacting growth and response mechanisms within cells.
Pathways
TSSC1 engages in intracellular transport and signal transduction pathways. It plays a significant role in the endosomal sorting pathway which controls material transport within cells. TSSC1 works closely with proteins like VPS26 and COMMD proteins which are essential players in endosomal sorting complexes. These interactions ensure efficient intracellular communication and materials handling affecting signal transduction pathways that mediate responses to external and internal signals.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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