Human TTC3 knockout HeLa cell lysate
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TTC3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon2.
View Alternative Names
DCRR1, E3 ubiquitin-protein ligase TTC3, Protein DCRR1, RING finger protein 105, RNF105, TPR repeat protein 3, TPR repeat protein D, TPRD, TPRDI, TPRDII, TPRDIII, TTC3_HUMAN, Tetratricopeptide repeat protein 3, Tetratricopeptide repeat protein 3 (TPR repeat protein D)
- Sanger seq
Unknown
Sanger Sequencing - Human TTC3 knockout HeLa cell lysate (AB258739)
Homozygous : Insertion of the selection cassette in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TTC3 interacts in various processes associated with cell development and stability. It is not part of a larger protein complex but operates notably in cell cycle progression and neuronal differentiation. TTC3 regulates the stability of proteins by adding ubiquitin molecules which are small regulatory proteins to target proteins. This activity is important for maintaining the cellular environment especially in tissues where precise control over protein levels is necessary.
Pathways
Many cellular pathways are influenced by TTC3 including the PI3K-AKT signaling pathway which mediates several cellular processes such as growth and survival. TTC3 has connections with proteins such as AKT1 through this pathway which helps regulate cell proliferation and neurogenesis. Additionally TTC3 is involved in the stability regulation of cyclin proteins influencing the cell cycle by interacting with components of the cyclin-dependent kinase (CDK) pathway therefore affecting cell cycle checkpoints and transitions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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