Human TTLL12 knockout HEK-293T cell lysate
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TTLL12 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 2.
View Alternative Names
FLJ41795, KIAA0153, TTL12_HUMAN, Tubulin tyrosine ligase like family, member 12, Tubulin--tyrosine ligase-like protein 12, dJ526I14.2
- Sanger seq
Unknown
Sanger Sequencing - Human TTLL12 knockout HEK-293T cell lysate (AB263401)
Homozygous : 1 bp deletion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TTLL12 influences neuronal and cellular functions through its capacity to alter the microtubule network. It participates in the polyglutamylation activity that regulates microtubule dynamics synaptic stability and intracellular transport playing critical roles in neurodevelopment and cellular organization. Although TTLL12 itself does not commonly form large complexes its enzymatic activity impacts other microtubule-modifying complexes indirectly affecting cellular architecture and signaling pathways.
Pathways
TTLL12 participates notably in the microtubule-network pathway and the regulation of cytoskeleton organization. Its activity modulates the polymerization and depolymerization dynamics of microtubules impacting cell cycle and neuronal signaling. TTLL12's function is related to other tubulin-modifying enzymes such as TTLL1 and TTLL5 which share a role in tubulin modification thereby affecting cellular processes like mitosis and intracellular trafficking.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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